A porphyrin-triazatruxene dyad for ratiometric two-photon fluorescent sensing of intracellular viscosity

By combining an electron-rich triazatruxene unit ( TAT ) to an electron-deficient zinc porphyrin fluorophore ( ZnPor ) via an ethynyl bridge, a new two-photon fluorescent viscosity rotor ( TAT-ZnPor ) with typical donor-π-acceptor (D--A) electronic configuration was developed for the ratiometric two...

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Bibliographic Details
Published in:Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2022-07, Vol.1 (28), p.5487-5492
Main Authors: Wang, Yi, Lei, Tong, Zhang, Jinghui, Gong, Lei, Yang, Yanjie, Ma, Xiaolin, Wen, Yongqiang, Du, Hongwu, Qi, Dongdong, Bian, Yongzhong, Liu, Zhiqiang, Jiang, Jianzhuang
Format: Article
Language:English
Subjects:
Absorption cross sections
Degeneration
Fluorescence
Glycerol
Intracellular
Mitochondria
Nystatin
Photon absorption
Photons
Porphyrins
Viscosity
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Summary:By combining an electron-rich triazatruxene unit ( TAT ) to an electron-deficient zinc porphyrin fluorophore ( ZnPor ) via an ethynyl bridge, a new two-photon fluorescent viscosity rotor ( TAT-ZnPor ) with typical donor-π-acceptor (D--A) electronic configuration was developed for the ratiometric two-photon fluorescent detection of intracellular viscosity. The TAT-ZnPor dyad exhibited highly improved fluorescence quantum yield ( Φ em = 0.40) and two-photon absorption cross-section ( δ TPA = 811 GM) in comparison to the individual components. In the methanol/glycerol system, TAT-ZnPor showed sensitive fluorescence responses toward the change of viscosity. Upon elevating the viscosity from 0.59 to 947 cp, the blue emission band around 410 nm gradually enhanced, while the red band at 647 nm concomitantly quenched, leading to a remarkable intensity ratio ( I 410 / I 647 ) change from 0.70 to 81 (116-fold). TAT-ZnPor also displayed good cell imaging performance under one- and two-photon excitation, and strong mitochondria targeting ability in living cells, thus was successfully applied in detecting the change of mitochondrial viscosity during the nystatin-induced degeneration. A new fluorescent viscosity rotor ( TAT-ZnPor ) shows highly improved two-photon action cross section and sensitive ratiometric response to mitochondrial viscosity in living cells.
ISSN:2050-750X
2050-7518
DOI:10.1039/d2tb00384h