β-Galactosidase-activated nitroxyl (HNO) donors provide insights into redox cross-talk in senescent cells

The cross-talk among reductive and oxidative species (redox cross-talk), especially those derived from sulfur, nitrogen and oxygen, influence several physiological processes including aging. One major hallmark of aging is cellular senescence, which is associated with chronic systemic inflammation. H...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2023-10, Vol.59 (85), p.12751-12754
Main Authors: Sawase, Laxman R, Kumar, T. Anand, Mathew, Abraham B, Khodade, Vinayak S, Toscano, John P, Saini, Deepak K, Chakrapani, Harinath
Format: Article
Language:English
Subjects:
Galactosidase
Hydrogen sulfide
Oxygen
Radiation effects
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Summary:The cross-talk among reductive and oxidative species (redox cross-talk), especially those derived from sulfur, nitrogen and oxygen, influence several physiological processes including aging. One major hallmark of aging is cellular senescence, which is associated with chronic systemic inflammation. Here, we report a chemical tool that generates nitoxyl (HNO) upon activation by β-galactosidase, an enzyme that is over-expressed in senescent cells. In a radiation-induced senescence model, the HNO donor suppressed reactive oxygen species (ROS) in a hydrogen sulfide (H 2 S)-dependent manner. Hence, the newly developed tool provides insights into redox cross-talk and establishes the foundation for new interventions that modulate levels of these species to mitigate oxidative stress and inflammation. A probe that generates nitroxyl (HNO) reveals the influence of redox cross-talk in cells.
ISSN:1359-7345
1364-548X
DOI:10.1039/d3cc03094f