Construction of a novel aminofluorene-based ratiometric near-infrared fluorescence probe for detecting carboxylesterase activity in living cells

Herein, we constructed a novel aminofluorene-based fluorescence probe ( FEN-CE ) for the detection of carboxylesterase (CE) in living cells by a ratiometric near-infrared (NIR) fluorescence signal. FEN-CE with NIR emission (650 nm) could be hydrolyzed specifically by CE and transformed to FENH with...

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Bibliographic Details
Published in:Analytical methods 2024-06, Vol.16 (23), p.3641-3645
Main Authors: Li, Jun-Mei, Liu, Yan-Zhao, Lv, Xiao-Fang, Zhou, Ding-Heng, Zhang, Hong, Chen, Yu-Jin, Li, Kun
Format: Article
Language:English
Subjects:
Carboxylesterase
Carboxylesterase - analysis
Carboxylesterase - metabolism
Cells (biology)
Emission
Emissions
Fluorenes - chemistry
Fluorescence
Fluorescent Dyes - chemistry
Fluorescent indicators
HeLa Cells
Humans
I.R. radiation
Near infrared radiation
Spectrometry, Fluorescence - methods
Spectroscopy, Near-Infrared - methods
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Summary:Herein, we constructed a novel aminofluorene-based fluorescence probe ( FEN-CE ) for the detection of carboxylesterase (CE) in living cells by a ratiometric near-infrared (NIR) fluorescence signal. FEN-CE with NIR emission (650 nm) could be hydrolyzed specifically by CE and transformed to FENH with the release of the self-immolative group, which exhibited a red-shifted emission peak of 680 nm. In addition, FEN-CE showed high selectivity for CE and was successfully used in the detection of CE activity in living cells through its ratiometric NIR fluorescence signals. Herein, we constructed a novel aminofluorene-based fluorescence probe ( FEN-CE ) for the detection of carboxylesterase (CE) in living cells by a ratiometric near-infrared (NIR) fluorescence signal.
ISSN:1759-9660
1759-9679
1759-9679
DOI:10.1039/d4ay00501e