A rhodamine based near-infrared fluorescent probe for selective detection of Cu 2+ ions and its applications in bioimaging

A novel near-infrared fluorescent probe TM2 based on a rhodamine-bearing framework was disclosed with a large Stokes shift (100 nm). TM2 exhibits highly selective recognition for Cu in EtOH/H O (1 : 1, v/v) solution with a low detection limit (2.3 μM) and a wide detection range (0-50 μM). Detection...

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Bibliographic Details
Published in:Analytical methods 2024-12, Vol.17 (1), p.178-183
Main Authors: Chang, Zhijian, Li, Shumeng, Ye, Jia-Hai, Lin, Fuyan, Chen, Yuncong, Guo, Zijian, Gong, Xuedong
Format: Article
Language:English
Subjects:
Animals
Copper - analysis
Copper - chemistry
Fluorescent Dyes - chemistry
HeLa Cells
Humans
Limit of Detection
Mice
Optical Imaging - methods
Rhodamines - chemistry
Spectrometry, Fluorescence - methods
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Summary:A novel near-infrared fluorescent probe TM2 based on a rhodamine-bearing framework was disclosed with a large Stokes shift (100 nm). TM2 exhibits highly selective recognition for Cu in EtOH/H O (1 : 1, v/v) solution with a low detection limit (2.3 μM) and a wide detection range (0-50 μM). Detection of Cu is undisturbed at physiological pH levels of 5-9. This recognition mechanism is attributed to the formation of a 1 : 1 complex between TM2 and Cu , validated by Job's plot, H NMR titration, and LC-MS experiments. Moreover, the successful fluorescence imaging of Cu both and was also accomplished.
ISSN:1759-9660
1759-9679
DOI:10.1039/d4ay01922a