Determination of chlortetracycline residues in tissues using high-performance liquid chromatography with fluorescence detection

A method is described for the determination of chlortetracycline residues in tissue samples. The samples were extracted into a hydrochloric acid - glycine solution and the extracts concentrated and purified on cyclohexyl-bonded reversed-phase cartridges. Any chlortetracycline present was converted t...

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Bibliographic Details
Published in:Analyst (London) 1989, Vol.114 (4), p.421-423
Main Authors: BLANCHFLOWER, W. J, MCCRACKEN, R. J, RICE, D. A
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chlortetracycline - analysis
Chromatography, High Pressure Liquid
Drug Residues - analysis
Food industries
Fundamental and applied biological sciences. Psychology
Meat and meat product industries
Spectrometry, Fluorescence
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Summary:A method is described for the determination of chlortetracycline residues in tissue samples. The samples were extracted into a hydrochloric acid - glycine solution and the extracts concentrated and purified on cyclohexyl-bonded reversed-phase cartridges. Any chlortetracycline present was converted to iso-chlortetracycline at pH 12, which was then separated from interfering compounds on a reversed-phase polymeric column using high-performance liquid chromatography with fluorescence detection. The detection and determination limits of the assay were 20 and 50 ng g-1, respectively, making it suitable for statutory residue testing purposes.
ISSN:0003-2654
1364-5528
DOI:10.1039/AN9891400421