Dendronised block copolymers as potential vectors for gene transfection

A series of block copolymers containing a dendronised cationic block for efficient DNA binding and a poly(ethylene glycol) block for encapsulation of the complex were synthesised in a modular fashion using a combination of click chemistry and ring-opening metathesis polymerisation. DNA binding exper...

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Bibliographic Details
Published in:Organic & biomolecular chemistry 2008-01, Vol.6 (11), p.1905
Main Authors: Wigglesworth, Tony J, Teixeira, Francisco, Axthelm, Fabian, Eisler, Sara, Csaba, Noemi S, Merkle, Hans P, Meier, Wolfgang, Diederich, François
Format: Article
Language:English
Subjects:
Dendrimers
Light
Microscopy, Electron, Transmission
Polymers - chemistry
Scattering, Radiation
Transfection
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Summary:A series of block copolymers containing a dendronised cationic block for efficient DNA binding and a poly(ethylene glycol) block for encapsulation of the complex were synthesised in a modular fashion using a combination of click chemistry and ring-opening metathesis polymerisation. DNA binding experiments, investigated using gel electrophoresis, dynamic light scattering and transmission electron microscopy, showed that all polymers prepared in this study strongly complex DNA and self-assemble into polyion complex micelles with apparent hydrodynamic radii ranging from 20-120 nm at physiological pH (7.4). The in vitro transfection efficiency and toxicity of these potential non-viral vectors were also evaluated in HeLadouble dagger cells using plasmid DNA encoding for green fluorescent protein as the reporter gene.
ISSN:1477-0520
1477-0539
DOI:10.1039/b802808g