In situ monitoring of antibiotic susceptibility of bacterial biofilms in a microfluidic device

Antibiotic resistance of biofilms is a growing public health concern due to overuse and improper use of antibiotics. Thus, determining an effective minimal concentration of antibiotics to eradicate bacterial biofilms is crucial. Here we present a simple, novel one-pot assay for the analysis of antib...

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Bibliographic Details
Published in:Lab on a chip 2010-01, Vol.1 (23), p.3296-3299
Main Authors: Kim, Keun Pil, Kim, Yun-Gon, Choi, Chang-Hyung, Kim, Hye-Eun, Lee, Sang-Ho, Chang, Woo-Suk, Lee, Chang-Soo
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Bacteria
Bacteria - metabolism
Biofilms
Kanamycin - pharmacology
Lab-On-A-Chip Devices
Microbial Sensitivity Tests
Microchip Analytical Procedures
Microfluidics
Plankton - metabolism
Pseudomonas aeruginosa
Quality Control
Reproducibility of Results
Rhodamines - chemistry
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Summary:Antibiotic resistance of biofilms is a growing public health concern due to overuse and improper use of antibiotics. Thus, determining an effective minimal concentration of antibiotics to eradicate bacterial biofilms is crucial. Here we present a simple, novel one-pot assay for the analysis of antibiotic susceptibility of bacterial biofilms using a microfluidics system where continuous concentration gradients of antibiotics are generated. The results of minimal biofilm eradication concentration (MBEC) clearly confirm that the concentration required to eradicate biofilm-grown Pseudomonas aeruginosa is higher than the minimal inhibitory concentration (MIC) that has been widely used to determine the lowest concentration of antibiotics against planktonically grown bacteria. This study presents a microfluidic approach for the in situ analysis of antibiotic susceptibility of bacterial biofilms using the generation of a continuous concentration gradient of antibiotics.
ISSN:1473-0197
1473-0189
DOI:10.1039/c0lc00154f