Expression and characterization of human interferon-β1 in the methylotrophic yeast Pichia pastoris

We describe the heterologous expression of a human interferon‐β1 in the methylotrophic yeast Pichia pastoris. Biologically active recombinant human interferon‐β1 (rHuIFN‐β1) was secreted from shake‐flask‐grown P. pastoris cells into the medium using the Saccharomyces cerevisiae α‐mating factor prepr...

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Bibliographic Details
Published in:Biotechnology and applied biochemistry 2003-12, Vol.38 (3), p.257-265
Main Authors: Skoko, Nataša, Argamante, Barbara, Grujičić, Nataša Kovačević, Tisminetzky, Sergio Gabriel, Glišin, Vladimir, Ljubijankić, Goran
Format: Article
Language:English
Subjects:
human interferon-β
KEX2
methylotrophic yeast
Pichia pastoris
α-mating factor
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Summary:We describe the heterologous expression of a human interferon‐β1 in the methylotrophic yeast Pichia pastoris. Biologically active recombinant human interferon‐β1 (rHuIFN‐β1) was secreted from shake‐flask‐grown P. pastoris cells into the medium using the Saccharomyces cerevisiae α‐mating factor prepro‐leader sequence at the level of (1–3)×105 i.u. (international units)/ml (6–12 mg/litre). An rHuIFN‐β1 with an N‐terminal sequence identical with that of native HuIFN‐β1 was purified and the specific activity was determined (2–3×107 i.u./mg). It was found that the secreted recombinant protein was partially N‐glycosylated.
ISSN:0885-4513
1470-8744
DOI:10.1042/BA20030065