Synergistic evaluation of AgO 2 nanoparticles with ceftriaxone against CTXM and blaSHV genes positive ESBL producing clinical strains of Uro-pathogenic E. coli

The silver oxide nanoparticles (AgO -NPs) were synthesised using silver foil as a new precursor in wet chemical method. X-ray diffraction analysis shows crystallographic structures of AgO -NPs with crystallite size of 35.54 nm well-matched with standard cubic structure. Scanning electron microscopy...

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Bibliographic Details
Published in:IET nanobiotechnology 2019-06, Vol.13 (4), p.435-440
Main Authors: Sajjad, Shamaila, Uzair, Bushra, Shaukat, Anum, Jamshed, Madiha, Leghari, Sajjad Ahmed Khan, Ismail, Muhammad, Mansoor, Qaiser
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Ceftriaxone - chemistry
Ceftriaxone - pharmacology
Drug Synergism
Escherichia coli - drug effects
Metal Nanoparticles - chemistry
Microbial Sensitivity Tests
Oxides - chemistry
Oxides - pharmacology
Silver Compounds - chemistry
Silver Compounds - pharmacology
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Summary:The silver oxide nanoparticles (AgO -NPs) were synthesised using silver foil as a new precursor in wet chemical method. X-ray diffraction analysis shows crystallographic structures of AgO -NPs with crystallite size of 35.54 nm well-matched with standard cubic structure. Scanning electron microscopy analysis clearly shows the random distribution of spherical-shaped nanoparticles. Energy dispersive X-ray analysis confirmed the purity of the samples as it shows no impurity element. Fourier transforms infra-red analysis confirmed the formation of AgO -NPs with the presence of Ag-O-Ag stretching bond. All the techniques also confirmed the loading of ceftriaxone drug on the surface of AgO -NPs. This study also described the effect of AgO -NPs having synergistic activity with lactam antibiotic i.e. ceftriaxone against ESBL generating . Among isolated strains of , 60.0% were found to be ESBL producer. The synergistic activities of AgO -NPs with ceftriaxone suggest that these combinations are effective against MDR-ESBL strains as evident by increase in zone sizes. The present study observed rise in MDR-ESBL with polymorphism of blaCTXM and blaSHV causing UTI infections in Pakistani population. The antibiotic and AgO -NPs synergistic effect can be used as an efficient approach to combat uro-pathogenic infections.
ISSN:1751-8741
1751-875X
DOI:10.1049/iet-nbt.2018.5415