Down-regulation and clinical significance of miR-7-2-3p in papillary thyroid carcinoma with multiple detecting methods

• Published in: IET systems biology 2019-10, Vol.13 (5), p.225-233
• Main Authors: Wu, Hua-yu, Wei, Yi, Pan, Shang-ling
• Format: Article
• Language: English
• Subjects: altered miRNA expression participates; authors examined expression levels; biochemistry; bioinformatics; biological tissues; cancer; Cell Line, Tumor; cellular biophysics; Disease Progression; Down-Regulation; function enrichment analysis; functions; Gene Expression Regulation, Neoplastic; genetics; Genomics; GSE40807; GSE55780; GSE62054; GSE73182; Humans; MicroRNAs - genetics; miR‐7‐2‐3p; molecular biophysics; papillary thyroid carcinoma tissues; proteins; Research Article; RNA; ROC Curve; Thyroid Cancer, Papillary - diagnosis; Thyroid Cancer, Papillary - genetics; Thyroid Cancer, Papillary - metabolism; Thyroid Cancer, Papillary - pathology; Transcription Factors - genetics; Transcription Factors - metabolism; tumours
• ISSN: 1751-8849; 1751-8857
• DOI: 10.1049/iet-syb.2019.0025

Altered miRNA expression participates in the biological progress of thyroid carcinoma and functions as a diagnostic marker or therapeutic agent. However, the role of miR-7-2-3p is currently unclear. The authors’ study was the first investigation of miR-7-2-3p expression level and diagnostic ability in several public databases. Potential target genes were obtained from DIANA Tools, and function enrichment analysis was then performed. Furthermore, the authors examined expression levels of potential targets in the Human Protein Atlas (HPA) and the Cancer Genome Atlas (TCGA). Finally, the potential transcription factors (TFs) were predicted by JASPAR. TCGA, GSE62054, GSE73182, GSE40807, and GSE55780 revealed that miR-7-2-3p expression in papillary thyroid carcinoma (PTC) tissues was notably lower compared with non-tumour tissues, while its expression in E-MATB-736 showed no remarkable difference. Function enrichment analysis showed that 698 genes were enriched in pathways, including pathways in cancer, and glioma. CCND1, GSK3B, and ITGAV of pathways in cancer were inverse correlations with miR-7-2-3p in both post-transcription and protein levels. According to the TF prediction, the prospective upstream TFs of miR-7-2-3p were ISX, SPI1, PRRX1, and BARX1. MiR-7-2-3p was significantly down-regulated and may act on PTC progression by crucial pathways. However, the mechanisms of miR-7-2-3p need further investigation.