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Serological diagnosis of toxoplasmosis: evaluation of the commercial test recom Line Toxoplasma IgG immunoblot (Mikrogen) based on recombinant antigens

Background : IgG detection to determine immune status to Toxoplasma gondii infection and seroconversion mainly relies on ELISA techniques and, if necessary, on a confirmatory test, Western blot. This study evaluated the performance of the recom Line Toxoplasma IgG immunoblot (IB- recom Line) (Mikrog...

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Bibliographic Details
Published in:Parasite (Paris) 2022, Vol.29, p.52
Main Authors: Jean-Pierre, Vincent, Miozzo, Julien, Fricker-Hidalgo, Hélène, Garnaud, Cécile, Robert, Marie Gladys, Pelloux, Hervé, Brenier-Pinchart, Marie-Pierre
Format: Article
Language:English
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Summary:Background : IgG detection to determine immune status to Toxoplasma gondii infection and seroconversion mainly relies on ELISA techniques and, if necessary, on a confirmatory test, Western blot. This study evaluated the performance of the recom Line Toxoplasma IgG immunoblot (IB- recom Line) (Mikrogen) as a confirmatory test on a large number of sera. A total of 171 sera were selected (113 patients) and had previously been analyzed by two ELISA tests, ARCHITECT (Abbott) and VIDAS (bioMérieux) ± LDBIO-Toxo II IgG Western blot (WB-LDBIO) (LDBio). The sera were classified into three groups: group 1 included 50 sera without difficulty in interpreting the IgG results (patients with documented past infection or uninfected); group 2 included 47 sera with difficulty in interpreting the ELISA results; and group 3 included 74 sequential sera from 25 pregnant women with seroconversion. Results : In group 1, overall IgG agreements were 94% and 90% with ARCHITECT and VIDAS, respectively. In group 2, low agreement was observed between IB- recom Line and WB-LDBIO, with eight false-positive and 13 false-negative results. In group 3, 4/13 seroconversions were detected earlier with IB- recom Line compared to other tests. Conclusions : IB- recom Line allowed for earlier diagnosis of toxoplasmic seroconversion compared to both ELISA tests and WB-LDBIO but led to insufficient performance to confirm the immune status when ELISA results were discordant or equivocal. Contexte  : La détection des IgG pour déterminer le statut immunitaire vis-à-vis de l’infection à Toxoplasma gondii et de la séroconversion repose principalement sur les techniques ELISA et, si nécessaire, sur un test de confirmation, le Western blot. Cette étude a évalué les performances de l’immunoblot recom Line Toxoplasma IgG (IB- recom Line) (Mikrogen) en tant que test de confirmation sur un grand nombre de sérums. Un total de 171 sérums ont été sélectionnés (113 patients) et ont été préalablement analysés par deux tests ELISA, ARCHITECT (Abbott) et VIDAS (bioMérieux) ± LDBIO-Toxo II IgG Western blot (WB-LDBIO) (LDBio). Les sérums ont été classés en trois groupes : le groupe 1 comprenait 50 sérums sans difficulté d’interprétation des résultats IgG (patients avec antécédents d’infection documentée ou non infectés); le groupe 2 comprenait 47 sérums avec des difficultés d’interprétation des résultats ELISA; le groupe 3 comprenait 74 sérums séquentiels de 25 femmes enceintes séroconverties. Résultats : Dans le group
ISSN:1776-1042
1776-1042
DOI:10.1051/parasite/2022050