In vitro Study of Pasteurella multocida Adhesion to Trachea, Lung and Aorta of Rabbits

In vitro experiments were undertaken to study the adhesion and colonization to tracheal mucosa, lung and aorta explants from freshly killed rabbits of two different strains of Pasteurella multocida. Serotype A:3 (capsulated, fimbriae +, haemagglutination –, dermonecrotic toxin –) isolated from a rab...

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Bibliographic Details
Published in:The veterinary journal (1997) 2000-05, Vol.159 (3), p.274-281
Main Authors: AL-HADDAWI, M.H., JASNI, S., ZAMRI-SAAD, M., MUTALIB, A.R., ZULKIFLI, I., SON, R., SHEIKH-OMAR, A.R.
Format: Article
Language:English
Subjects:
Adhesion
Animals
Aorta - microbiology
Aorta - ultrastructure
Bacterial Adhesion
Culture Techniques
Dermotoxins - metabolism
Erythrocytes - microbiology
explant
Humans
in vitro
Lung - microbiology
Lung - ultrastructure
Microscopy, Electron, Scanning
Pasteurella Infections - microbiology
Pasteurella Infections - veterinary
Pasteurella multocida
Pasteurella multocida - classification
Pasteurella multocida - pathogenicity
Pasteurella multocida - physiology
rabbit
Rabbits - microbiology
Trachea - microbiology
Trachea - ultrastructure
Virulence
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Summary:In vitro experiments were undertaken to study the adhesion and colonization to tracheal mucosa, lung and aorta explants from freshly killed rabbits of two different strains of Pasteurella multocida. Serotype A:3 (capsulated, fimbriae +, haemagglutination –, dermonecrotic toxin –) isolated from a rabbit with rhinitis, and serotype D:1 (non-capsulated, fimbriae +, haemagglutination +, dermonecrotic toxin +) isolated from a dead rabbit with septicaemia, were used. When the explants were observed under the scanning electron microscope, the type D strain was highly adherent to trachea and aorta explants compared to the type A strain. Adhesion to lung explants was best achieved by the type A strain after 45 min incubation, but after 2 h incubation no significant difference was observed between the strains. Our data indicate that the presence of fimbriae and the absence of capsule seem to enhance the adherence of P. multocida type D strain to tracheal tissue. The capsular material of P. multocida type A strain and the toxin of the type D strain seem to influence the adherence to lung tissue in rabbit. Adhesion of strain D to aorta may indicate the expression of receptors on the endothelium to that strain and may also explain the ability of certain strains to cause septicaemia.
ISSN:1090-0233
1532-2971
DOI:10.1053/tvjl.1999.0418