Ibuprofen attenuates early lung injury in endotoxemic, neutropenic rats

The objective of our study was to determine the role of ibuprofen in protecting neutropenic rats from cardiopulmonary injury due to endotoxemia. We hypothesized that ibuprofen would offer pulmonary protection by altering cytokine production. Neutropenic rats received E. coli lipopolysaccharide (LPS)...

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Bibliographic Details
Published in:Prostaglandins, leukotrienes and essential fatty acids leukotrienes and essential fatty acids, 2001-08, Vol.65 (2), p.59-65
Main Authors: Daphtary, K.M., Heidemann, S.M., Glibetic, M.
Format: Article
Language:English
Subjects:
Animals
Anti-Inflammatory Agents, Non-Steroidal - pharmacology
Biological and medical sciences
Bones, joints and connective tissue. Antiinflammatory agents
Bronchoalveolar Lavage Fluid
Chemokine CXCL2
Chemokines - biosynthesis
Cyclophosphamide - pharmacology
Cytokines - metabolism
Endotoxemia - drug therapy
Hemodynamics
Humans
Ibuprofen - pharmacology
Inflammation
Lung - metabolism
Male
Medical sciences
Neutropenia - drug therapy
Neutrophils - metabolism
Nitric Oxide Synthase - metabolism
Peroxidase - metabolism
Pharmacology. Drug treatments
Rats
Rats, Sprague-Dawley
RNA, Messenger - metabolism
Time Factors
Tumor Necrosis Factor-alpha - biosynthesis
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Summary:The objective of our study was to determine the role of ibuprofen in protecting neutropenic rats from cardiopulmonary injury due to endotoxemia. We hypothesized that ibuprofen would offer pulmonary protection by altering cytokine production. Neutropenic rats received E. coli lipopolysaccharide (LPS) alone or ibuprofen and LPS. After 4 h, arterial blood gases, heart rate and blood pressure were measured. Blood and bronchoalveolar lavage fluid (BALF) were collected for TNF-α and MIP-2 concentrations. Lung tissue for iNOS mRNA and myeloperoxidase were obtained. The ibuprofen group had decreased heart rate and better oxygenation. Ibuprofen suppressed TNF- α and MIP-2 production in blood and MIP-2 concentrations in BALF. Lung mRNA for iNOS was higher in the ibuprofen group. Neutrophil infiltration in the lung was similar in both groups. Ibuprofen attenuated cardiopulmonary dysfunction by decreasing the early cytokine response. The balance of vasodilator to vasoconstrictor production in the lung may favor vasodilation as shown by increased iNOS mRNA and suppression of thromboxane.
ISSN:0952-3278
1532-2823
DOI:10.1054/plef.2001.0289