Synthesis of an Azo-Cyanine Dye as an RNA Probe for Distinguishing Gram-Positive Bacteria from Gram-Negative Bacteria

We have developed a new cyanine fluorescent probe, Azo-ETO3, that shows better RNA selectivity than commercial dyes in solution. Azo-ETO3 can image the RNA of mitochondria in living cells and the RNA of the nucleolus and cytoplasm in fixed cells. As a practical application, Azo-ETO3 emits strong flu...

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Bibliographic Details
Published in:Synlett 2023-03, Vol.35 (1), p.95-100
Main Authors: Wu, Ruixin, Li, Zipeng, Xia, Tianping, Cai, Wenlin, Du, Jianjun, Fan, Jiangli, Peng, Xiaojun
Format: Article
Language:English
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Summary:We have developed a new cyanine fluorescent probe, Azo-ETO3, that shows better RNA selectivity than commercial dyes in solution. Azo-ETO3 can image the RNA of mitochondria in living cells and the RNA of the nucleolus and cytoplasm in fixed cells. As a practical application, Azo-ETO3 emits strong fluorescence when interacting with Gram-positive bacteria, and it can be used to selectively label G+ bacteria in the presence of other bacteria. In addition, Azo-ETO3 exhibits low toxicity and has essentially no major impact on bacterial colony growth. These properties could make it useful as a tool for distinguishing bacterial species.
ISSN:0936-5214
1437-2096
DOI:10.1055/s-0042-1751462