Monitoring cell-cycle-related viscoelasticity by a quartz crystal microbalance

We have monitored viscoelasticity variation of a cell population during the cell cycle by a Quartz Crystal Microbalance (QCM). Balb 3T3 fibroblasts were synchronized in the G0/G1 phase and seeded in a QCM chamber placed in a cell incubator. After cell sedimentation, the frequency signal was characte...

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Bibliographic Details
Published in:Applied physics letters 2006-02, Vol.88 (8), p.083905-083905-3
Main Authors: Alessandrini, A., Croce, M. A., Tiozzo, R., Facci, P.
Format: Article
Language:English
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Summary:We have monitored viscoelasticity variation of a cell population during the cell cycle by a Quartz Crystal Microbalance (QCM). Balb 3T3 fibroblasts were synchronized in the G0/G1 phase and seeded in a QCM chamber placed in a cell incubator. After cell sedimentation, the frequency signal was characterized by an amplitude modulation attributed to the viscoelasticity variation of the cells proliferating in phase. A control experiment with nonsynchronized cells showed a similar signal trend, but without significant modulation. Interestingly, the system resulted also to perform as a device sensitive to the effect of drugs affecting the cell cycle, such as colchicine.
ISSN:0003-6951
1077-3118
DOI:10.1063/1.2177647