Construction of multiphoton laser scanning microscope based on dual-axis acousto-optic deflector

The construction of a multiphoton laser scanning microscope based on a dual-axis acousto-optic deflector (AOD) is described. Without mechanical inertia and physical movement of the scanner, this system has a high random-addressing and data-acquisition rate of 10 μ s pixel . By proper compensation of...

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Bibliographic Details
Published in:Review of scientific instruments 2006-04, Vol.77 (4), p.046101-046101-3
Main Authors: Lv, Xiaohua, Zhan, Chen, Zeng, Shaoqun, Chen, Wei R., Luo, Qingming
Format: Article
Language:English
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Summary:The construction of a multiphoton laser scanning microscope based on a dual-axis acousto-optic deflector (AOD) is described. Without mechanical inertia and physical movement of the scanner, this system has a high random-addressing and data-acquisition rate of 10 μ s pixel . By proper compensation of the dispersion of the AODs, the spatial resolution of the system is 0.5 μ m for lateral direction and 1.5 μ m for axial direction. Fluorescence images are acquired to demonstrate these imaging performances.
ISSN:0034-6748
1089-7623
DOI:10.1063/1.2190047