Method for measuring the three-dimensional distribution of a fluorescent dye in a cell membrane

This letter reports on a method for accurately determining the component distribution in a cell membrane over the entire cell surface. This method involves exciting a fluorescent-dyed cell membrane using evanescent light and scanning the entire cell surface by rotating the cell using a noncontact te...

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Bibliographic Details
Published in:Applied physics letters 2007-01, Vol.90 (2), p.021110-021110-3
Main Authors: Yamamoto, Kazuya, Ishimaru, Ichirou, Fujii, Yoshiki, Yasokawa, Toshiki, Kuriyama, Shigeki, Masaki, Tsutomu, Takegawa, Kaoru, Tanaka, Naotaka
Format: Article
Language:English
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Summary:This letter reports on a method for accurately determining the component distribution in a cell membrane over the entire cell surface. This method involves exciting a fluorescent-dyed cell membrane using evanescent light and scanning the entire cell surface by rotating the cell using a noncontact technique, namely, proximal two-beam optical tweezers. To position the cell membrane in the thin evanescent field, the authors designed an optical system capable of precisely positioning the focal position. Using this method, they were able to measure the surface distribution of glycoprotein labeled by lectin in a breast cancer cell membrane.
ISSN:0003-6951
1077-3118
DOI:10.1063/1.2428457