Self-formation of bilayer lipid membranes on agarose-coated silicon surfaces studied by simultaneous electrophysiological and surface infrared spectroscopic measurements

Self-formation process of bilayer lipid membranes (BLMs) cushioned on agarose-coated Si surfaces was in situ monitored by simultaneous electrophysiological and infrared absorption spectroscopic (IRAS) measurements using IRAS with the multiple internal reflection geometry. IRAS signals corresponding...

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Bibliographic Details
Published in:Applied physics letters 2009-06, Vol.94 (24), p.243906-243906-3
Main Authors: Hirano-Iwata, Ayumi, Oshima, Azusa, Onodera, Kota, Aoto, Kouji, Taira, Tasuku, Yamaguchi, Ryo-taro, Kimura, Yasuo, Niwano, Michio
Format: Article
Language:English
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Summary:Self-formation process of bilayer lipid membranes (BLMs) cushioned on agarose-coated Si surfaces was in situ monitored by simultaneous electrophysiological and infrared absorption spectroscopic (IRAS) measurements using IRAS with the multiple internal reflection geometry. IRAS signals corresponding to self-thinning of lipid solution to form BLMs were demonstrated. It was found that the appearance of IRAS bands due to C = O modes of phosphstidylcholine is related to formation of BLMs with a gigaohm seal. The functionality of the present BLM system was also demonstrated by incorporating gramicidin into the BLMs and recording its channel activities.
ISSN:0003-6951
1077-3118
DOI:10.1063/1.3140206