Detection of organophosphorus compound based on a sol–gel silica planar waveguide doped with a green fluorescent protein and an organophosphorus hydrolase

In this letter, the authors report the real-time detection of an organophosphorus compound using a sol–gel silica planar waveguide doped with a green fluorescent protein and an organophosphorus hydrolase on a yeast-cell surface display. The waveguide was pumped at 488 nm, and it emitted green fluore...

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Bibliographic Details
Published in:Applied physics letters 2011-06, Vol.98 (23)
Main Authors: Enami, Y., Tsuchiya, K., Suye, S.
Format: Article
Language:English
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Summary:In this letter, the authors report the real-time detection of an organophosphorus compound using a sol–gel silica planar waveguide doped with a green fluorescent protein and an organophosphorus hydrolase on a yeast-cell surface display. The waveguide was pumped at 488 nm, and it emitted green fluorescence at the far field. The green fluorescent light at 550 nm changed by 50% from the original power 1 min after application of the organophosphorus compound. The results enable the real-time detection of sarin and other biochemicals by using an in-line fiber sensor network.
ISSN:0003-6951
1077-3118
DOI:10.1063/1.3596448