Time-resolved hyperspectral fluorescence spectroscopy using frequency-modulated excitation

An intensity-modulated excitation light source is used together with a micro-channel plate intensified CCD detector gated at a slightly different frequency to generate a beat frequency from a fluorescent sample. The addition of a spectrograph produces a hyperspectral time-resolved data product where...

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Bibliographic Details
Published in:Journal of applied physics 2012-07, Vol.112 (1)
Main Authors: DiBenedetto, John, Capelle, Gene A., O’Neill, Mary
Format: Article
Language:English
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Summary:An intensity-modulated excitation light source is used together with a micro-channel plate intensified CCD detector gated at a slightly different frequency to generate a beat frequency from a fluorescent sample. The addition of a spectrograph produces a hyperspectral time-resolved data product where the resulting beat frequency is detected with a low frame rate camera. Measuring the beat frequency of the spectrum as a function of time allows separation of the excited fluorescence from ambient constant light sources. The excitation and detector repetition rates are varied over a range of discrete frequencies, and the phase shift of the beat wave maps out the emission decay rate(s).
ISSN:0021-8979
1089-7550
DOI:10.1063/1.4731247