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A practical wide-field Raman imaging method with high spectral and spatial resolution

Raman imaging has a great advantage in characterizing inhomogeneous systems. A practical wide-field Raman imaging platform is developed that shows major improvements on imaging speed, sensitivity, and resolution. Different from the traditional Raman imaging systems using the wavelength-fixed lasers...

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Bibliographic Details
Published in:Review of scientific instruments 2018-08, Vol.89 (8), p.083103-083103
Main Authors: Li, Haibo, Luo, Wenhua, Li, Gan, Zhang, Guangfeng, Zhang, Pengcheng, Li, Changmao, Gu, Yuejiao
Format: Article
Language:English
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Summary:Raman imaging has a great advantage in characterizing inhomogeneous systems. A practical wide-field Raman imaging platform is developed that shows major improvements on imaging speed, sensitivity, and resolution. Different from the traditional Raman imaging systems using the wavelength-fixed lasers and the chromatic dispersion devices, this system adopts an inverted architecture, integrated with a tunable laser and the wavelength-fixed filters. Owing to the high transmission of the fixed filters, the imaging sensitivity can be improved 5-10 times in comparison to the present wide-field Raman imaging setups using liquid-crystal tunable filters. Via combining with the high-power tunable laser, Raman images could be obtained in minutes and the Raman shift of the images could be tuned easily and accurately. The resolution of this system can reach 1.5 cm−1 in the spectrum and 490 nm in space, which could provide more fingerprint details of the analytes. This effective Raman imaging method allowing us to see chemical spatial variations on microscale is anticipated to be widely applied in scientific research fields.
ISSN:0034-6748
1089-7623
DOI:10.1063/1.5041529