Damage on Escherichia coli and Staphylococcus aureus using white light photoactivation of Au and Ag nanoparticles

• Published in: Journal of applied physics 2019-06, Vol.125 (21)
• Main Authors: Méndez-Pfeiffer, Pablo A., Soto Urzúa, Lucia, Sánchez-Mora, Enrique, González, Ana L., Romo-Herrera, J. M., Gervacio Arciniega, José Juan, Martínez Morales, Luis Javier
• Format: Article
• Language: English
• Subjects: Applied physics; Bacteria; Cetyltrimethylammonium bromide; Damage assessment; E coli; Gold; Nanoparticles; Penicillin; Silver; Size distribution; Surface roughness; Viability; White light
• ISSN: 0021-8979; 1089-7550
• DOI: 10.1063/1.5090273

Bactericidal efficiency of Au and Ag nanoparticles (NPs) is reported with and without photoactivation by white light. Au and Ag NPs were synthesized with an average size of 14 ± 1.2 nm and of 4.6 ± 0.5 nm, respectively. The size distribution of the Ag colloid was relatively wide. Less than 4 % of these NPs were largely decahedral, which, based on numerical calculations, determined the position of the optical band. In contrast, the Au colloid had a narrow optical band; a concentration of 1.3 μ g / ml was determined by theoretical and experimental spectra. Ag and Au NPs showed a superficial charge of − 35 mV and + 57 mV due to the presence of the citrate ions and cetyltrimethylammonium bromide on their surface, respectively. The effect of the NPs concentration on the viability of Escherichia coli and Staphylococcus aureus strains was investigated. It was found that Ag NPs were more effective against E. coli than Au NPs, whereas Au NPs were more effective against S. aureus than Ag NPs. The induced damage to the bacteria by the NPs was evaluated by AFM. The images show that the bacterial cell wall was changed in shape and in surface roughness, being more noticeable in S. aureus than in E. coli. The bactericidal activity of the photoactivated Ag NPs was almost doubled for both bacteria, whereas for the Au NPs, no bactericidal enhancement was observed for either strain. This can be explained by the high efficiency of Ag NPs to absorb white light and the consequent creation of hot spots that contribute to kill the bacteria.