Carbon nanotube substrates enhance SARS-CoV-2 spike protein ion yields in matrix-assisted laser desorption–ionization mass spectrometry

Nanostructured surfaces enhance ion yields in matrix-assisted laser desorption–ionization mass spectrometry (MALDI-MS). The spike protein complex, S1, is one fingerprint signature of Sars-CoV-2 with a mass of 75 kDa. Here, we show that MALDI-MS yields of Sars-CoV-2 spike protein ions in the 100 kDa...

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Bibliographic Details
Published in:Applied physics letters 2023-01, Vol.122 (5)
Main Authors: Schenkel, T., Snijders, A. M., Nakamura, K., Seidl, P. A., Mak, B., Obst-Huebl, L., Knobel, H., Pong, I., Persaud, A., van Tilborg, J., Ostermayr, T., Steinke, S., Blakely, E. A., Ji, Q., Javey, A., Kapadia, R., Geddes, C. G. R., Esarey, E.
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Applied physics
Desorption
Environmental monitoring
Ionization
laser ablation
Mass spectrometry
Multi wall carbon nanotubes
Nanostructure
nanostructures
nanotubes
Proteins
Severe acute respiratory syndrome coronavirus 2
Spectroscopy
Substrates
surface and interface chemistry
time of flight mass spectrometry
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Summary:Nanostructured surfaces enhance ion yields in matrix-assisted laser desorption–ionization mass spectrometry (MALDI-MS). The spike protein complex, S1, is one fingerprint signature of Sars-CoV-2 with a mass of 75 kDa. Here, we show that MALDI-MS yields of Sars-CoV-2 spike protein ions in the 100 kDa range are enhanced 50-fold when the matrix–analyte solution is placed on substrates that are coated with a dense forest of multi-walled carbon nanotubes, compared to yields from uncoated substrates. Nanostructured substrates can support the development of mass spectrometry techniques for sensitive pathogen detection and environmental monitoring.
ISSN:0003-6951
1077-3118
DOI:10.1063/5.0128667