Differentiation of Australian cucumber mosaic cucumovirus isolates: comparison of dsRNA type, polymerase chain reaction-restriction enzyme analysis, DNA hybridisation and serogrouping

We examined the differentiation of 22 Australian isolates of cucumber mosaic cucumovirus (CMV) by comparing PAGE mobilities of the three RNAs (dsRNA mobilities), serogroups using monoclonal antibodies, dot blot hybridisation (DBH) with subgroup-specific RNA 3 probes and PCR-restriction enzyme analys...

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Bibliographic Details
Published in:Australasian plant pathology 1998-01, Vol.27 (1), p.36-39
Main Authors: Pares, R.D., Dietzgen, R.G., Gunn, L.V., Rizos, H.
Format: Article
Language:English
Subjects:
Coat protein
Cucumis sativus
Cucumovirus
Cytomegalovirus
Differentiation
DNA
DNA probes
Double-stranded RNA
Enzymes
Mobility
Monoclonal antibodies
Mosaics
RNA
RNA probes
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Summary:We examined the differentiation of 22 Australian isolates of cucumber mosaic cucumovirus (CMV) by comparing PAGE mobilities of the three RNAs (dsRNA mobilities), serogroups using monoclonal antibodies, dot blot hybridisation (DBH) with subgroup-specific RNA 3 probes and PCR-restriction enzyme analysis (Msp I) of the coat protein gene. While there was a high degree of correlation between the results obtained with all four methods, we found exceptions with 2 of the 22 isolates analysed. This indicates that more than one test is necessary to obtain reliable information about the identity of a CMV isolate Australasian Plant Pathology 27(1) 36 - 39 Full text doi:10.1071/AP98002 © CSIRO 1998
ISSN:0815-3191
0156-0972
1448-6032
DOI:10.1071/AP98002