The DNA/RNA-Binding Protein MSY2 Marks Specific Transcripts for Cytoplasmic Storage in Mouse Male Germ Cells

During spermatogenesis, male germ cells temporally synthesize many proteins as they differentiate through meiosis and become spermatozoa. The germ cell Y-box protein, MSY2, constituting ≈0.7% of total protein in male germ cells, binds to a consensus promoter element, and shows a general lack of RNA-...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2005-02, Vol.102 (5), p.1513-1518
Main Authors: Yang, Juxiang, Medvedev, Sergey, Reddi, P. Prabhakara, Schultz, Richard M., Hecht, Norman B., Yanagimachi, Ryuzo
Format: Article
Language:English
Subjects:
Animals
Binding sites
Biological Sciences
Cell cycle
Cell growth
Cellular biology
Chromatin - physiology
Chromatin - ultrastructure
Cytoplasm - physiology
Deoxyribonucleic acid
DNA
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Genes
Germ cells
Immunoprecipitation
Male
Meiosis
Messenger RNA
Mice
Oocytes
Promoter Regions, Genetic - genetics
Protein Biosynthesis
Proteins
Recombinant Proteins - metabolism
Reverse Transcriptase Polymerase Chain Reaction
Ribonucleic acid
RNA
RNA, Messenger - genetics
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
Spermatozoa - cytology
Spermatozoa - physiology
Testes
Transcription, Genetic
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Summary:During spermatogenesis, male germ cells temporally synthesize many proteins as they differentiate through meiosis and become spermatozoa. The germ cell Y-box protein, MSY2, constituting ≈0.7% of total protein in male germ cells, binds to a consensus promoter element, and shows a general lack of RNA-binding specificity. Combining immunoprecipitation and suppressive subtractive hybridization, we identified populations of germ cell mRNAs that are not bound or bound by MSY2. The former population is enriched in cell growth and ubiquitously expressed mRNAs, whereas the latter population is enriched for stored or translationally delayed, male gamete-specific transcripts. Chromatin precipitation assays reveal that most of the MSY2 target mRNAs are transcribed from genes containing the Y-box DNA-binding motif in their promoters. In transgenic mice, mRNAs encoding exogenous GFP are directed or not directed into the MSY2-bound fraction by promoters containing or lacking the Y-box motif, respectively. We propose that MSY2 marks specific mRNAs in the nucleus for cytoplasmic storage, thereby linking transcription and mRNA storage/translational delay in meiotic and postmeiotic male germ cells of the mouse.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0404685102