Adapter Proteins SLP-76 and BLNK Both Are Expressed by Murine Macrophages and Are Linked to Signaling via Fcγ Receptors I and II/III

The SLP-76 (Src homology 2 domain-containing leukocyte protein of 76 kDa) adapter protein is expressed in T cells and myeloid cells, whereas its homologue BLNK (B cell linker protein) is expressed in B cells. SLP-76 and BLNK link immunoreceptor tyrosine-based activation motif-containing receptors to...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2000-02, Vol.97 (4), p.1725-1730
Main Authors: Bonilla, Francisco A., Fujita, Ross M., Pivniouk, Vadim I., Chan, Andrew C., Geha, Raif S.
Format: Article
Language:English
Subjects:
B lymphocytes
Biological Sciences
BLNK protein
Bone marrow cells
Crosslinking
Fcg receptors
Ligation
Macrophages
Mice
Phagocytosis
Phosphorylation
Ras protein
Receptors
Rho protein
SLP-76 protein
T lymphocytes
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Summary:The SLP-76 (Src homology 2 domain-containing leukocyte protein of 76 kDa) adapter protein is expressed in T cells and myeloid cells, whereas its homologue BLNK (B cell linker protein) is expressed in B cells. SLP-76 and BLNK link immunoreceptor tyrosine-based activation motif-containing receptors to signaling molecules that include phospholipase C-γ , mitogen-activated protein kinases, and the GTPases Ras and Rho. SLP-76 plays a critical role in T cell receptor, Fcε RI and gpVI collagen receptor signaling, and participates in signaling via Fcγ R and killer cell inhibitory receptors. BLNK plays a critical role in B cell receptor signaling. We show that murine bone marrow-derived macrophages express both SLP-76 and BLNK. Selective ligation of Fcγ RI and Fcγ RII/III resulted in tyrosine phosphorylation of both SLP-76 and BLNK. SLP-76-/-bone marrow-derived macrophages display Fcγ R-mediated tyrosine phosphorylation of Syk, phospholipase C-γ 2, and extracellular signal regulated kinases 1 and 2, and normal Fcγ R-dependent phagocytosis. These data suggest that both SLP-76 and BLNK are coupled to Fcγ R signaling in murine macrophages.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.040543597