Early Expression of Antiinsulin Autoantibodies of Humans and the NOD Mouse: Evidence for Early Determination of Subsequent Diabetes

With the development of an insulin autoantibody (IAA) assay performed in 96-well filtration plates, we have evaluated prospectively the development of IAA in NOD mice (from 4 weeks of age) and children (from 7 to 10 months of age) at genetic risk for the development of type 1 diabetes. NOD mice had...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2000-02, Vol.97 (4), p.1701-1706
Main Authors: Yu, Liping, Robles, David T., Abiru, Norio, Kaur, Paramjit, Rewers, Marian, Kelemen, Katalin, Eisenbarth, George S.
Format: Article
Language:English
Subjects:
Age Factors
Animals
Autoantibodies
Autoantibodies - blood
Biological Sciences
Blood Glucose - analysis
Child development
Child, Preschool
Children
Developmental biology
Diabetes
Diabetes complications
Diabetes Mellitus, Type 1 - blood
Diabetes Mellitus, Type 1 - immunology
Female
Follow-Up Studies
Generally accepted auditing standards
Genetics
Humans
Immunoassay
Immunology
Infant
Insulin
Insulin antibodies
Insulin Antibodies - blood
Insulin Antibodies - immunology
Male
Mice
Mice, Inbred NOD
Risk Factors
Time Factors
Type 1 diabetes mellitus
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Summary:With the development of an insulin autoantibody (IAA) assay performed in 96-well filtration plates, we have evaluated prospectively the development of IAA in NOD mice (from 4 weeks of age) and children (from 7 to 10 months of age) at genetic risk for the development of type 1 diabetes. NOD mice had heterogeneous expression of IAA despite being inbred. IAA reached a peak between 8 and 16 weeks and then declined. IAA expression by NOD mice at 8 weeks of age was strongly associated with early development of diabetes, which occurred at 16-18 weeks of age (NOD mice IAA+at 8 weeks: 83% (5/6) diabetic by 18 weeks versus 11% (1/9) of IAA negative at 8 weeks; P
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.040556697