Salt Tolerance of Arabidopsis thaliana Requires Maturation of N-Glycosylated Proteins in the Golgi Apparatus

Protein N-glycosylation in the endoplasmic reticulum (ER) and in the Golgi apparatus is an essential process in eukaryotic cells. Although the N-glycosylation pathway in the ER has been shown to regulate protein quality control, salt tolerance, and cellulose biosynthesis in plants, no biological rol...

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Published in:Proceedings of the National Academy of Sciences - PNAS 2008-04, Vol.105 (15), p.5933-5938
Main Authors: Kang, Jae Sook, Frank, Julia, Kang, Chang Ho, Kajiura, Hiroyuki, Vikram, Meenu, Ueda, Akihiro, Kim, Sewon, Bahk, Jeong Dong, Triplett, Barbara, Fujiyama, Kazuhito, Lee, Sang Yeol, von Schaewen, Antje, Koiwa, Hisashi
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Arabidopsis - chemistry
Arabidopsis - physiology
Arabidopsis Proteins - biosynthesis
Arabidopsis thaliana
Biological Sciences
Biosynthesis
Botany
Cells
Flowers & plants
Genetic mutation
Genetics
Glycoproteins
Glycoproteins - biosynthesis
Glycosylation
Golgi apparatus
Golgi Apparatus - metabolism
Mutation
Phenotypes
Plant cells
Plants
Root growth
Salt tolerance
Salts
Seedlings
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Summary:Protein N-glycosylation in the endoplasmic reticulum (ER) and in the Golgi apparatus is an essential process in eukaryotic cells. Although the N-glycosylation pathway in the ER has been shown to regulate protein quality control, salt tolerance, and cellulose biosynthesis in plants, no biological roles have been linked functionally to N-glycan modifications that occur in the Golgi apparatus. Herein, we provide evidence that mutants defective in N-glycan maturation, such as complex glycan 1 (cgl1), are more salt-sensitive than wild type. Salt stress caused growth inhibition, aberrant root-tip morphology, and callose accumulation in cgl1, which were also observed in an ER oligosaccharyltransferase mutant, staurosporin and temperature sensitive 3a (stt3a). Unlike stt3a, cgl1 did not cause constitutive activation of the unfolded protein response. Instead, aberrant modification of the plasma membrane glycoprotein KORRIGAN 1/RADIALLY SWOLLEN 2 (KOR1/RSW2) that is necessary for cellulose biosynthesis occurred in cgl1 and stt3a. Genetic analyses identified specific interactions among rsw2, stt3a, and cgl1 mutations, indicating that the function of KOR1/RSW2 protein depends on complex N-glycans. Furthermore, cellulose deficient rsw1-1 and rsw2-1 plants were also salt-sensitive. These results establish that plant protein N-glycosylation functions beyond protein folding in the ER and is necessary for sufficient cell-wall formation under salt stress.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0800237105