CentO satellite confers translational and rotational phasing on cenH3 nucleosomes in rice centromeres

Plant and animal centromeres comprise megabases of highly repeated satellite sequences, yet centromere function can be specified epigenetically on single-copy DNA by the presence of nucleosomes containing a centromere-specific variant of histone H3 (cenH3). We determined the positions of cenH3 nucle...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2013-12, Vol.110 (50), p.E4875-E4883
Main Authors: Zhang, Tao, Talbert, Paul B, Zhang, Wenli, Wu, Yufeng, Yang, Zujun, Henikoff, Jorja G, Henikoff, Steven, Jiang, Jiming
Format: Article
Language:English
Subjects:
animals
Biological Sciences
Centromere - genetics
Centromere - metabolism
centromeres
Chromatin Immunoprecipitation
Chromosomes
DNA
Epigenesis, Genetic - genetics
Epigenetics
Evolution, Molecular
histones
Histones - genetics
Histones - metabolism
micrococcal nuclease
Micrococcal Nuclease - metabolism
nucleosomes
Nucleosomes - genetics
Nucleosomes - metabolism
Oryza - genetics
Oryza - metabolism
Oryza sativa
periodicity
PNAS Plus
Proteins
Rice
Satellite DNA
satellites
Sequence Analysis, DNA
Tandem Repeat Sequences - genetics
translation (genetics)
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Summary:Plant and animal centromeres comprise megabases of highly repeated satellite sequences, yet centromere function can be specified epigenetically on single-copy DNA by the presence of nucleosomes containing a centromere-specific variant of histone H3 (cenH3). We determined the positions of cenH3 nucleosomes in rice (Oryza sativa), which has centromeres composed of both the 155-bp CentO satellite repeat and single-copy non- CentO sequences. We find that cenH3 nucleosomes protect 90–100 bp of DNA from micrococcal nuclease digestion, sufficient for only a single wrap of DNA around the cenH3 nucleosome core. cenH3 nucleosomes are translationally phased with 155-bp periodicity on CentO repeats, but not on non- CentO sequences. CentO repeats have an ∼10-bp periodicity in WW dinucleotides and in micrococcal nuclease cleavage, providing evidence for rotational phasing of cenH3 nucleosomes on CentO and suggesting that satellites evolve for translational and rotational stabilization of centromeric nucleosomes.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1319548110