Convex lens-induced nanoscale templating

Significance Convex lens-induced nanoscale templating (CLINT) represents a conceptual breakthrough in nanofluidic technology for single-molecule manipulation. CLINT solves a key challenge faced by the nanofluidics field by bridging the multiple-length scales required to efficiently bring single-mole...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2014-09, Vol.111 (37), p.13295-13300
Main Authors: Berard, Daniel J., Michaud, François, Mahshid, Sara, Ahamed, Mohammed Jalal, McFaul, Christopher M. J., Leith, Jason S., Bérubé, Pierre, Sladek, Rob, Reisner, Walter, Leslie, Sabrina R.
Format: Article
Language:English
Subjects:
Biological Sciences
Deoxyribonucleic acid
DNA
DNA - chemistry
Dyes
Electric fields
Fluorescence
Genomics
Imaging
Imaging, Three-Dimensional
Lenses
Molecules
Nanofluidics
Nanomaterials
Nanostructures - chemistry
Nanotechnology - methods
Nucleic Acid Denaturation
Physical Sciences
Polymers
Research fellowships
Topography
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Summary:Significance Convex lens-induced nanoscale templating (CLINT) represents a conceptual breakthrough in nanofluidic technology for single-molecule manipulation. CLINT solves a key challenge faced by the nanofluidics field by bridging the multiple-length scales required to efficiently bring single-molecule analytes from the pipette tip to the nanofluidic channel. To do this, CLINT loads single-molecule analytes into embedded nanofeatures via dynamic control of applied vertical confinement, which we have demonstrated by loading and extending DNA within nanochannels. CLINT offers unique advantages in single-molecule DNA mapping by facilitating surface passivation, increasing loading efficiency, obviating the need for applied pressure or electric fields, and enhancing compatibility with physiological buffers and long DNA molecules extracted from complex genomes.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1321089111