Functional magnetic resonance microscopy at single-cell resolution in Aplysia californica

In this work, we show the feasibility of performing functional MRI studies with single-cell resolution. At ultrahigh magnetic field, manganese-enhanced magnetic resonance microscopy allows the identification of most motor neurons in the buccal network of Aplysia at low, nontoxic Mn ²⁺ concentrations...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2014-06, Vol.111 (23), p.8667-8672
Main Authors: Radecki, Guillaume, Nargeot, Romuald, Jelescu, Ileana Ozana, Le Bihan, Denis, Ciobanu, Luisa
Format: Article
Language:English
Subjects:
Animals
Aplysia - cytology
Aplysia - metabolism
Aplysia - physiology
Appetitive Behavior - physiology
Behavioral neuroscience
Biological Sciences
Brain research
Feasibility Studies
Feeding Behavior - physiology
Food
Ganglia
Ganglia, Invertebrate - cytology
Ganglia, Invertebrate - metabolism
Imaging
Magnetic resonance imaging
Magnetic Resonance Imaging - methods
Manganese - metabolism
Manganese - pharmacokinetics
Mental stimulation
Microscopy
Motor Neurons - metabolism
Motor Neurons - physiology
Neurons
Neurosciences
NMR
Nuclear magnetic resonance
Physiological stimulation
Reproducibility of Results
Single-Cell Analysis - methods
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Summary:In this work, we show the feasibility of performing functional MRI studies with single-cell resolution. At ultrahigh magnetic field, manganese-enhanced magnetic resonance microscopy allows the identification of most motor neurons in the buccal network of Aplysia at low, nontoxic Mn ²⁺ concentrations. We establish that Mn ²⁺ accumulates intracellularly on injection into the living Aplysia and that its concentration increases when the animals are presented with a sensory stimulus. We also show that we can distinguish between neuronal activities elicited by different types of stimuli. This method opens up a new avenue into probing the functional organization and plasticity of neuronal networks involved in goal-directed behaviors with single-cell resolution.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1403739111