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Functional magnetic resonance microscopy at single-cell resolution in Aplysia californica

In this work, we show the feasibility of performing functional MRI studies with single-cell resolution. At ultrahigh magnetic field, manganese-enhanced magnetic resonance microscopy allows the identification of most motor neurons in the buccal network of Aplysia at low, nontoxic Mn ²⁺ concentrations...

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Published in:	Proceedings of the National Academy of Sciences - PNAS 2014-06, Vol.111 (23), p.8667-8672
Main Authors:	Radecki, Guillaume, Nargeot, Romuald, Jelescu, Ileana Ozana, Le Bihan, Denis, Ciobanu, Luisa
Format:	Article
Language:	English
Subjects:	Animals Aplysia - cytology Aplysia - metabolism Aplysia - physiology Appetitive Behavior - physiology Behavioral neuroscience Biological Sciences Brain research Feasibility Studies Feeding Behavior - physiology Food Ganglia Ganglia, Invertebrate - cytology Ganglia, Invertebrate - metabolism Imaging Magnetic resonance imaging Magnetic Resonance Imaging - methods Manganese - metabolism Manganese - pharmacokinetics Mental stimulation Microscopy Motor Neurons - metabolism Motor Neurons - physiology Neurons Neurosciences NMR Nuclear magnetic resonance Physiological stimulation Reproducibility of Results Single-Cell Analysis - methods
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creator	Radecki, Guillaume Nargeot, Romuald Jelescu, Ileana Ozana Le Bihan, Denis Ciobanu, Luisa
description	In this work, we show the feasibility of performing functional MRI studies with single-cell resolution. At ultrahigh magnetic field, manganese-enhanced magnetic resonance microscopy allows the identification of most motor neurons in the buccal network of Aplysia at low, nontoxic Mn ²⁺ concentrations. We establish that Mn ²⁺ accumulates intracellularly on injection into the living Aplysia and that its concentration increases when the animals are presented with a sensory stimulus. We also show that we can distinguish between neuronal activities elicited by different types of stimuli. This method opens up a new avenue into probing the functional organization and plasticity of neuronal networks involved in goal-directed behaviors with single-cell resolution.
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subjects	Animals Aplysia - cytology Aplysia - metabolism Aplysia - physiology Appetitive Behavior - physiology Behavioral neuroscience Biological Sciences Brain research Feasibility Studies Feeding Behavior - physiology Food Ganglia Ganglia, Invertebrate - cytology Ganglia, Invertebrate - metabolism Imaging Magnetic resonance imaging Magnetic Resonance Imaging - methods Manganese - metabolism Manganese - pharmacokinetics Mental stimulation Microscopy Motor Neurons - metabolism Motor Neurons - physiology Neurons Neurosciences NMR Nuclear magnetic resonance Physiological stimulation Reproducibility of Results Single-Cell Analysis - methods
title	Functional magnetic resonance microscopy at single-cell resolution in Aplysia californica
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