Induction of Colonies of Hemoglobin-Synthesizing Cells by Erythropoietin In Vitro

A culture method has been developed in which erythroid colonies are produced in vitro from hemopoietic cells from the livers of 13-day fetuses of C3Hf/Bi mice. Heme synthesis by the cultures was correlated with the presence of these colonies, and the hemoglobin produced was shown to be electrophoret...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1971-07, Vol.68 (7), p.1542-1546
Main Authors: Stephenson, John R., Axelrad, Arthur A., McLeod, David L., Shreeve, Mona M.
Format: Article
Language:English
Subjects:
Animals
Autoradiography
Biological Sciences: Medical Sciences
Blood plasma
Bone marrow
Bone Marrow Cells
Cell culture techniques
Cell Line - drug effects
Citrates
Culture Techniques
Cultured cells
Electrophoresis
Erythrocytes - analysis
Erythroid cells
Erythropoietin - pharmacology
Female
Fetus
Gels
Hemoglobins
Hemoglobins - analysis
Hemoglobins - biosynthesis
Iron
Iron Isotopes
Leukocytes
Liver
Liver cells
Male
Mice
Peroxidases - analysis
Staining and Labeling
Time Factors
Transferrin
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A culture method has been developed in which erythroid colonies are produced in vitro from hemopoietic cells from the livers of 13-day fetuses of C3Hf/Bi mice. Heme synthesis by the cultures was correlated with the presence of these colonies, and the hemoglobin produced was shown to be electrophoretically normal. The individual colonies were identified as erythroid since they were erythropoietin-dependent, positively stained by the histochemical `Lepehne' procedure for hemoglobin, and labeled by59Fe radioautography. Evidence is presented that the development of these colonies is under separate control from that of granulocytic colonies found in the same cultures.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.68.7.1542