The Stabilization of Episomal Integration by Genetic Inversion: A General Hypothesis

In F′cells of Escherichia coli K12, recombination in the region of genetic duplication between chromosome and F-genote produces a rapid alteration between the integrated and detached states of the episome. Nevertheless, F′strains can give rise to Hfr cells in which the F-genote has undergone stable...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1972-08, Vol.69 (8), p.2061-2065
Main Authors: Adelberg, Edward A., Bergquist, Peter
Format: Article
Language:English
Subjects:
Biological Sciences: Genetics
Cells
Chromosome Aberrations
Chromosomes
Chromosomes, Bacterial
Coliphages
Conjugation, Genetic
Crossing Over, Genetic
DNA
Escherichia coli - enzymology
Escherichia coli - metabolism
Escherichia coli - radiation effects
Extrachromosomal Inheritance
Fucose - metabolism
Galactose - metabolism
Genes
Genetic mutation
Glucose
Isomerases - biosynthesis
Lysogeny
Medical genetics
Microbial genetics
Models, Biological
Operons
Plasmids
Radiation Effects
Recombination, Genetic
Region of integration
Ultraviolet Rays
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Summary:In F′cells of Escherichia coli K12, recombination in the region of genetic duplication between chromosome and F-genote produces a rapid alteration between the integrated and detached states of the episome. Nevertheless, F′strains can give rise to Hfr cells in which the F-genote has undergone stable integration. We have found that one such Hfr has retained the genetic duplication as well as the recombination proficiency of the parental F′strain; to account for the failure of recombination to detach the episome in this Hfr, we have postulated the inversion of one of the duplicated regions. A general model is presented, showing that an inversion must prevent episome detachment by any combination of genetic crossovers.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.69.8.2061