The Membrane Junctions in Communicating and Noncommunicating Cells, their Hybrids, and Segregants

Human Lesch-Nyhan cells, which are coupling and have gap junctions, were fused with mouse cl-1D cells, which are noncoupling and lack gap junctions. The resulting hybrid cells were coupling and had gap junctions while they contained the nearly complete complement of parent chromosomes. As the hybrid...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1974-03, Vol.71 (3), p.880-884
Main Authors: Azarnia, R., Larsen, W. J., Loewenstein, W. R.
Format: Article
Language:English
Subjects:
Animals
Apposition
Biological Sciences: Cell Biology
Cell Line
Cell membranes
Cells
Cellular differentiation
Clone Cells
Embryonic cells
Fluoresceins
Gap junctions
Humans
Hybrid cells
Hybrid Cells - cytology
Hybridity
Intercellular Junctions
Karyotyping
L cells
L Cells (Cell Line)
Lesch-Nyhan Syndrome
Membrane Potentials
Mice
Microscopy, Electron
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Summary:Human Lesch-Nyhan cells, which are coupling and have gap junctions, were fused with mouse cl-1D cells, which are noncoupling and lack gap junctions. The resulting hybrid cells were coupling and had gap junctions while they contained the nearly complete complement of parent chromosomes. As the hybrid cells lost human chromosomes, clones appeared among the segregants, which had reverted to the noncoupling and junction-deficient trait of the mouse parent cell. The human cell appears to contribute a genetic factor to the hybrids that corrects the junctional deficiency of the mouse cell.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.71.3.880