Partial Amino Acid Sequence of Penicillinase Coded by Escherichia coli Plasmid R6K

Direct studies of the amino acid sequence of an Escherichia coli plasmid-coded penicillinase (penicillin amido-β -lactamhydrolase, EC 3.5.2.6) are in complete agreement with results derived from the translation of the DNA sequence of a related plasmid, apart from a single amino acid substitution. Th...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1978-08, Vol.75 (8), p.3732-3736
Main Authors: Ambler, R. P., Scott, G. K.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
beta-Lactamases - genetics
Biochemistry
DNA
Enzymes
Escherichia coli
Escherichia coli - genetics
Gram positive bacteria
Isothiocyanates
Nucleotide sequences
Penicillin
Penicillinase - biosynthesis
Penicillinase - genetics
Plasmids
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Summary:Direct studies of the amino acid sequence of an Escherichia coli plasmid-coded penicillinase (penicillin amido-β -lactamhydrolase, EC 3.5.2.6) are in complete agreement with results derived from the translation of the DNA sequence of a related plasmid, apart from a single amino acid substitution. This penicillinase from a Gram-negative bacterium shows 30-35% identity with functionally similar enzymes from Gram-positive bacteria. This paper should be read in conjunction with the report of the DNA sequence of the gene [Sutcliffe, J. G. (1978) Proc. Natl. Acad. Sci. USA 75, 3737-3741].
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.75.8.3732