Induction of Amplified Synthesis and Secretion of IgM by Fusion of Murine B Lymphoma with Myeloma Cells

Murine B lymphoma cultured cell lines bearing membrane IgM and lacking the ability to secrete measurable amounts of IgM were fused with drug-resistant cell lines derived from the IgG2b-producing MPC-11 myeloma. Many of the hybrid clones synthesized and secreted large amounts of IgM, as judged by rad...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1979-02, Vol.76 (2), p.915-919
Main Authors: Laskov, Reuven, Kim, K. Jin, Asofsky, Richard
Format: Article
Language:English
Subjects:
Animals
B cell lymphoma
B lymphocytes
B-Lymphocytes - cytology
B-Lymphocytes - immunology
B-Lymphocytes - metabolism
Cell Differentiation
Cell lines
Cells, Cultured
Chromosomes
Cytoplasm - metabolism
Electrophoresis
Gels
Hybrid cells
Hybrid Cells - immunology
Hybridity
Immunoglobulin M - metabolism
Karyotyping
Lymphoma
Mice
Neoplasms, Experimental - immunology
Plasmacytoma - immunology
Receptors, Antigen, B-Cell - metabolism
Secretion
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Summary:Murine B lymphoma cultured cell lines bearing membrane IgM and lacking the ability to secrete measurable amounts of IgM were fused with drug-resistant cell lines derived from the IgG2b-producing MPC-11 myeloma. Many of the hybrid clones synthesized and secreted large amounts of IgM, as judged by radial and double immunodiffusion in agar of culture supernatants and by polyacrylamide gel electrophoresis of biosynthetically labeled IgM. When fused with an IgG2b-producing myeloma, many of the hybrids also produced IgG2b, indicating that there was no suppression of the parental IgG synthesis in the hybrids. The amount of IgM or IgG2bsecreted by the hybrids was higher than that secreted by myeloma cell lines. The synthesis of the γ2bheavy chain of the MPC-11 myeloma was not reexpressed by fusion of a γ2bnonproducer myeloma cell variant with B lymphoma. No other classes of Ig heavy chains, besides the γ2band the μ chains, were found to be secreted by any of more than 100 B lymphoma-myeloma hybrids examined. The results of the present work suggest that myeloma cells may fuse not only with normal plasma cells but also with less-mature normal B lymphocytes and that this fusion ``induces'' the maturation of B lymphocytes into IgM-secreting cells.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.76.2.915