Purification of Human Platelet-Derived Growth Factor

Human platelets contain a polypeptide growth factor that stimulates the proliferation of connective tissue cells. Purification of this platelet-derived growth factor (PDGF) was accomplished by heat (100 degrees C) treatment of washed platelets and subsequent ion-exchange chromatography, gel filtrati...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1979-04, Vol.76 (4), p.1809-1813
Main Authors: Antoniades, Harry N., Scher, Charles D., Stiles, Charles D.
Format: Article
Language:English
Subjects:
3T3 cells
Blood Platelets - analysis
Cell Division - drug effects
Cell growth
Chromatography
DNA
Electrophoresis
Filtration
Gels
Growth Substances - blood
Growth Substances - isolation & purification
Growth Substances - pharmacology
Humans
Molecular Weight
Platelets
Sodium
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Summary:Human platelets contain a polypeptide growth factor that stimulates the proliferation of connective tissue cells. Purification of this platelet-derived growth factor (PDGF) was accomplished by heat (100 degrees C) treatment of washed platelets and subsequent ion-exchange chromatography, gel filtration in 1 M acetic acid, isoelectric focusing, and preparative sodium dodecyl sulfate/polyacrylamide gel electrophoresis. PDGF has an isoelectric point of 9.8 and a molecular weight ranging from 13,000 to 16,000 as judged by gel filtration in 1 M acetic acid or analytical sodium dodecyl sulfate gel electrophoresis under reducing conditions. The specific activity of the purified PDGF is 20 million times greater than that found in unfractionated human serum. Purified PDGF stimulates replicative DNA synthesis and cell proliferation in quiescent density-arrested cultures of BALB/c 3T3 cells at concentrations of 1 ng/ml (0.1 nM).
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.76.4.1809