Transcriptional Regulation of the Yeast Cytochrome c Gene

DNA from the cloned yeast iso-1-cytochrome c, cyc1, gene was used in a hybridization assay to measure levels and rates of synthesis of cyc1 RNA. Derepressed cells synthesized cyc1 RNA at 6 times the rate of that of glucose-repressed cells. Upon glucose addition to a derepressed culture, the transcri...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1979-08, Vol.76 (8), p.3627-3631
Main Authors: Zitomer, Richard S., Montgomery, Donna L., Nichols, Diane L., Hall, Benjamin D.
Format: Article
Language:English
Subjects:
Cell growth
Cytochrome c Group - genetics
Cytochromes
DNA
DNA, Recombinant
Genes
Glucose - metabolism
Half lives
Messenger RNA
Nucleic Acid Hybridization
Plasmids
Raffinose - metabolism
Repression
RNA
RNA, Messenger - metabolism
Saccharomyces cerevisiae - genetics
Transcription, Genetic
Yeasts
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Description
Summary:DNA from the cloned yeast iso-1-cytochrome c, cyc1, gene was used in a hybridization assay to measure levels and rates of synthesis of cyc1 RNA. Derepressed cells synthesized cyc1 RNA at 6 times the rate of that of glucose-repressed cells. Upon glucose addition to a derepressed culture, the transcription of the cyc1 gene was repressed within 2.5 min. The half-life of hybridizable cyc1 RNA was determined to be 12-13.5 min under repressed and derepressed conditions and during repression. The results demonstrate that the expression of the cyc1 gene is subject to transcriptional regulation.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.76.8.3627