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Transcriptional Regulation of the Yeast Cytochrome c Gene
DNA from the cloned yeast iso-1-cytochrome c, cyc1, gene was used in a hybridization assay to measure levels and rates of synthesis of cyc1 RNA. Derepressed cells synthesized cyc1 RNA at 6 times the rate of that of glucose-repressed cells. Upon glucose addition to a derepressed culture, the transcri...
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Published in: | Proceedings of the National Academy of Sciences - PNAS 1979-08, Vol.76 (8), p.3627-3631 |
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container_end_page | 3631 |
container_issue | 8 |
container_start_page | 3627 |
container_title | Proceedings of the National Academy of Sciences - PNAS |
container_volume | 76 |
creator | Zitomer, Richard S. Montgomery, Donna L. Nichols, Diane L. Hall, Benjamin D. |
description | DNA from the cloned yeast iso-1-cytochrome c, cyc1, gene was used in a hybridization assay to measure levels and rates of synthesis of cyc1 RNA. Derepressed cells synthesized cyc1 RNA at 6 times the rate of that of glucose-repressed cells. Upon glucose addition to a derepressed culture, the transcription of the cyc1 gene was repressed within 2.5 min. The half-life of hybridizable cyc1 RNA was determined to be 12-13.5 min under repressed and derepressed conditions and during repression. The results demonstrate that the expression of the cyc1 gene is subject to transcriptional regulation. |
doi_str_mv | 10.1073/pnas.76.8.3627 |
format | article |
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Derepressed cells synthesized cyc1 RNA at 6 times the rate of that of glucose-repressed cells. Upon glucose addition to a derepressed culture, the transcription of the cyc1 gene was repressed within 2.5 min. The half-life of hybridizable cyc1 RNA was determined to be 12-13.5 min under repressed and derepressed conditions and during repression. 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Derepressed cells synthesized cyc1 RNA at 6 times the rate of that of glucose-repressed cells. Upon glucose addition to a derepressed culture, the transcription of the cyc1 gene was repressed within 2.5 min. The half-life of hybridizable cyc1 RNA was determined to be 12-13.5 min under repressed and derepressed conditions and during repression. The results demonstrate that the expression of the cyc1 gene is subject to transcriptional regulation.</description><subject>Cell growth</subject><subject>Cytochrome c Group - genetics</subject><subject>Cytochromes</subject><subject>DNA</subject><subject>DNA, Recombinant</subject><subject>Genes</subject><subject>Glucose - metabolism</subject><subject>Half lives</subject><subject>Messenger RNA</subject><subject>Nucleic Acid Hybridization</subject><subject>Plasmids</subject><subject>Raffinose - metabolism</subject><subject>Repression</subject><subject>RNA</subject><subject>RNA, Messenger - metabolism</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Transcription, Genetic</subject><subject>Yeasts</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><recordid>eNp9kE1P3DAQhi1UaLfAtYeqSDn1ljD-iD8OPVSrQishISE4cLK8zoQNysZb20Hw75to6YpeehpZ7_PMWC8hnyhUFBQ_3w4uVUpWuuKSqQOyoGBoKYWBd2QBwFSpBRMfyMeUHgHA1BrekyPGpFFsQcxtdEPysdvmLgyuL27wYezd_ChCW-Q1FvfoUi6WLzn4dQwbLHxxiQOekMPW9QlPX-cxubv4cbv8WV5dX_5afr8qfc1oLmkDZgWN0dphK6QHbGit24Z5rXgtfSs8NGrVKMERhXIKwQnUFCXnUHvDj8m33d7tuNpg43HI0fV2G7uNiy82uM7-mwzd2j6EJ8s117qe_K-vfgy_R0zZbrrkse_dgGFMVglV61qxCax2oI8hpYjt_gYFO1dt56qtklbbuepJ-PL2Z3t81-2bw7P2N9zrth37PuNznsCz_4FT_nmXP6Yc4h6QRirJ_wBro5wM</recordid><startdate>19790801</startdate><enddate>19790801</enddate><creator>Zitomer, Richard S.</creator><creator>Montgomery, Donna L.</creator><creator>Nichols, Diane L.</creator><creator>Hall, Benjamin D.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19790801</creationdate><title>Transcriptional Regulation of the Yeast Cytochrome c Gene</title><author>Zitomer, Richard S. ; Montgomery, Donna L. ; Nichols, Diane L. ; Hall, Benjamin D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c521t-1d09b0d988aef46c0ed158fd2c87356cf4c0d7bd743ee47a7e0a4e81e63305c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Cell growth</topic><topic>Cytochrome c Group - genetics</topic><topic>Cytochromes</topic><topic>DNA</topic><topic>DNA, Recombinant</topic><topic>Genes</topic><topic>Glucose - metabolism</topic><topic>Half lives</topic><topic>Messenger RNA</topic><topic>Nucleic Acid Hybridization</topic><topic>Plasmids</topic><topic>Raffinose - metabolism</topic><topic>Repression</topic><topic>RNA</topic><topic>RNA, Messenger - metabolism</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Transcription, Genetic</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zitomer, Richard S.</creatorcontrib><creatorcontrib>Montgomery, Donna L.</creatorcontrib><creatorcontrib>Nichols, Diane L.</creatorcontrib><creatorcontrib>Hall, Benjamin D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zitomer, Richard S.</au><au>Montgomery, Donna L.</au><au>Nichols, Diane L.</au><au>Hall, Benjamin D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptional Regulation of the Yeast Cytochrome c Gene</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1979-08-01</date><risdate>1979</risdate><volume>76</volume><issue>8</issue><spage>3627</spage><epage>3631</epage><pages>3627-3631</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>DNA from the cloned yeast iso-1-cytochrome c, cyc1, gene was used in a hybridization assay to measure levels and rates of synthesis of cyc1 RNA. Derepressed cells synthesized cyc1 RNA at 6 times the rate of that of glucose-repressed cells. Upon glucose addition to a derepressed culture, the transcription of the cyc1 gene was repressed within 2.5 min. The half-life of hybridizable cyc1 RNA was determined to be 12-13.5 min under repressed and derepressed conditions and during repression. The results demonstrate that the expression of the cyc1 gene is subject to transcriptional regulation.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>226972</pmid><doi>10.1073/pnas.76.8.3627</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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language | eng |
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source | PubMed (Medline); JSTOR Archival Journals and Primary Sources Collection |
subjects | Cell growth Cytochrome c Group - genetics Cytochromes DNA DNA, Recombinant Genes Glucose - metabolism Half lives Messenger RNA Nucleic Acid Hybridization Plasmids Raffinose - metabolism Repression RNA RNA, Messenger - metabolism Saccharomyces cerevisiae - genetics Transcription, Genetic Yeasts |
title | Transcriptional Regulation of the Yeast Cytochrome c Gene |
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