Cloning of Integrated Moloney Sarcoma Proviral DNA Sequences in Bacteriophage λ

• Published in: Proceedings of the National Academy of Sciences - PNAS 1979-09, Vol.76 (9), p.4464-4468
• Main Authors: G. F. Vande Woude, Oskarsson, M., Enquist, L. W., Nomura, S., Sullivan, M., Fischinger, P. J.
• Format: Article
• Language: English
• Subjects: Animals; Bacteriophage lambda - genetics; Bacteriophages; Cells; Cells, Cultured; DNA; DNA probes; DNA Restriction Enzymes - metabolism; DNA, Recombinant; DNA, Viral - genetics; Genes, Viral; Genomes; Hybridity; Lungs; Mink; Moloney murine leukemia virus - genetics; Nucleotide sequences; Recombination, Genetic; RNA; Viruses
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.76.9.4464

We have identified integrated proviral DNA sequences of m1 and HT-1 isolates of Moloney sarcoma virus (MuSV) in EcoRI digests of transformed mink cell genomic DNA and have cloned these fragments in bacteriophage λ . Both the λ -HT1 phage recombinant, containing a 12.3-kilobase MuSV pair (kb) fragment, and the λ -ml phage recombinant, containing a 7.0-kb fragment, possess full copies of the sarcoma viruses along with 5′and 3′host flanking sequences. The MuSV proviral DNA sequences, 6.7 kb for HT-1 and 5.2 kb for m1, are colinear by heteroduplex microscopy with the 1.5-kb difference in size accounted for by two ≈ 0.8-kb deleted regions in m1. Both integrated viral genomes are terminally redundant and have integrated at the same site in the provirus but at different sites on the host chromosome. The host sequences flanking integrated HT-1 MuSV have been identified as a single EcoRI restriction fragment of 5.6 kb in normal mink cells.