Translation and Developmental Regulation of RNA Encoded by the Eukaryotic Transposable Element Copia

copia-specific RNA was isolated from Drosophila melanogaster tissue culture cells by hybridization of cytoplasmic polyadenylylated RNA to copia DNA immobilized on cellulose. The purified RNA was translated in reticulocyte lysates. One major polypeptide of approximately 51,000 daltons was synthesized...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1980-12, Vol.77 (12), p.7107-7111
Main Authors: Flavell, Andrew J., Ruby, Stephanie W., Toole, John J., Roberts, Bryan E., Rubin, Gerald M.
Format: Article
Language:English
Subjects:
Animals
Cell culture techniques
Cultured cells
DNA
DNA Transposable Elements
Drosophila
Drosophila melanogaster - embryology
Drosophila melanogaster - genetics
Drosophila melanogaster - growth & development
Gels
Genes
Messenger RNA
Molecular Weight
Plasmids
Protein Biosynthesis
Proteins - genetics
RNA
RNA, Messenger - genetics
Tissue culture techniques
Transcription, Genetic
Transposons
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Description
Summary:copia-specific RNA was isolated from Drosophila melanogaster tissue culture cells by hybridization of cytoplasmic polyadenylylated RNA to copia DNA immobilized on cellulose. The purified RNA was translated in reticulocyte lysates. One major polypeptide of approximately 51,000 daltons was synthesized in addition to several others between 18,000 and 38,000 daltons. The 51,000-dalton polypeptide and several of the others are encoded by mRNAs of about 2000 nucleotides. The approximate locations on the copia element of the coding sequences for the 51,000-dalton polypeptide and several other proteins were determined by hybrid-arrested translation with copia restriction fragments. The relative abundance of copia-specific RNA was determined at various stages of the Drosophila life cycle. The level of copia-specific RNA is modulated during development of the organism, with the highest level occurring during the larval stages.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.77.12.7107