Isolation of a cDNA Clone for Mouse Urinary Proteins: Age- And Sex-Related Expression of Mouse Urinary Protein Genes is Transcriptionally Controlled

A recombinant cDNA plasmid derived from mouse urinary protein (MUP) mRNA was isolated and used to determine the level of control of the developmentally regulated and the sex-linked expression of MUP genes by monitoring the transcription of MUP mRNA sequences in isolated liver nuclei. No transcriptio...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1981-09, Vol.78 (9), p.5425-5429
Main Author: Derman, Eva
Format: Article
Language:English
Subjects:
Adult animals
Age Factors
Animals
Cellulose nitrate
Cloning, Molecular - methods
DNA
Female
Gene Expression Regulation
Genes
Immatures
Liver
Liver - growth & development
Liver - metabolism
Male
Male animals
Messenger RNA
Mice
Plasmids
Protein Biosynthesis
Proteins - genetics
Proteinuria
RNA
RNA, Messenger - genetics
Sex Factors
Transcription, Genetic
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Summary:A recombinant cDNA plasmid derived from mouse urinary protein (MUP) mRNA was isolated and used to determine the level of control of the developmentally regulated and the sex-linked expression of MUP genes by monitoring the transcription of MUP mRNA sequences in isolated liver nuclei. No transcription of MUP genes could be detected in liver nuclei of prepubescent animals whose livers do not contain measurable MUP mRNA. Transcription of MUP genes in the livers of adult male mice was 6-fold higher than in the livers of adult female mice, proportional to the difference in MUP mRNA concentrations. Transcriptional control mechanisms are therefore implicated as responsible for both the developmentally and the sex-linked changes in the expression of MUP genes.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.78.9.5425