Absence of Pork-Like Insulin in Guinea Pig Tissues

By using a technique for concentrating insulin 100-fold from tissue extracts with 75-95% recoveries, we earlier failed to detect pork-like insulin in guinea pig tissues and thus were unable to confirm reports from the National Institutes of Health that these tissues contain a pork-like insulin at co...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1982-04, Vol.79 (8), p.2683-2685
Main Authors: Eng, John, Yalow, Rosalyn S.
Format: Article
Language:English
Subjects:
Animals
Antiserum
Brain Chemistry
Guinea pigs
Guinea Pigs - metabolism
Insulin
Insulin - analysis
Kidney - analysis
Kidneys
Messenger RNA
Pork
Radioimmunoassay
Rats
Species Specificity
Swine
Tissue extracts
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Summary:By using a technique for concentrating insulin 100-fold from tissue extracts with 75-95% recoveries, we earlier failed to detect pork-like insulin in guinea pig tissues and thus were unable to confirm reports from the National Institutes of Health that these tissues contain a pork-like insulin at concentrations averaging 1 ng/g. This difference could have been due to differences in strains of guinea pigs studied or in the species specificities of the antisera used for radioimmunoassay. In the current study, tissue extracts from both NIH and Hartley guinea pigs were assayed with three antisera routinely used in our laboratory and one antiserum that had been used in the National Institutes of Health laboratory. We observed that pork-like insulin in tissues from both strains of guinea pigs as determined with the four antisera is
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.79.8.2683