Distinct Organization of Methylcholanthrene- and Phenobarbital-Inducible Cytochrome P-450 Genes in the Rat

The complete nucleotide sequence of the methylcholanthrene-inducible cytochrome P-450c gene was determined by sequence analysis of cloned genomic DNA and the sequence, consisting of 524 amino acids, of the protein was deduced therefrom. The gene for the cytochrome was approximately 6.0 kilobases lon...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1984-08, Vol.81 (16), p.5066-5070
Main Authors: Sogawa, Kazuhiro, Gotoh, Osamu, Kawajiri, Kaname, Fujii-Kuriyama, Yoshiaki
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
Base Sequence
Biochemistry
Complementary DNA
Cytochrome P-450 Enzyme System - biosynthesis
Cytochrome P-450 Enzyme System - genetics
Cytochromes
DNA
DNA - isolation & purification
DNA Restriction Enzymes
Enzyme Induction
Genes - drug effects
Genomics
Introns
Messenger RNA
Methylcholanthrene - pharmacology
Nucleic Acid Hybridization
Nucleotide sequences
Phenobarbital - pharmacology
Plasmids
Rats
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Summary:The complete nucleotide sequence of the methylcholanthrene-inducible cytochrome P-450c gene was determined by sequence analysis of cloned genomic DNA and the sequence, consisting of 524 amino acids, of the protein was deduced therefrom. The gene for the cytochrome was approximately 6.0 kilobases long and was split into seven exons. Comparison of the gene with that of the phenobarbital-inducible cytochrome P-450e showed that the gene structures for the two types of cytochrome P-450 differ greatly; the location, number, and size of intervening sequences are very dissimilar. However, the sequence homology between the two types of cytochrome suggests that the two genes have evolved from a common ancestor.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.81.16.5066