Cloning, characterization, and sequence of the yeast DNA topoisomerase I gene

The structural gene for yeast DNA topoisomerase I (TOP1) has been cloned from two yeast genomic plasmid banks. Integration of a plasmid carrying the gene into the chromosome and subsequent genetic mapping shows that TOP1 is identical to the gene previously called MAK1. Seven top1 (mak1) mutants incl...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1985-07, Vol.82 (13), p.4374-4378
Main Authors: Thrash, C, Bankier, A.T, Barrell, B.G, Sternglanz, R
Format: Article
Language:English
Subjects:
ADN
Amino acids
Base Sequence
Biological and medical sciences
Biotechnology
CLONACION
CLONAGE
CLONING
Cloning, Molecular
Deoxyribonuclease HindIII
Diploidy
DNA
DNA Restriction Enzymes - metabolism
DNA Topoisomerases, Type I - genetics
Electrophoresis, Agar Gel
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genetic engineering
Genetic mutation
Genetic technics
ISOMERASAS
ISOMERASE
ISOMERASES
Methods. Procedures. Technologies
Molecular cloning
MUTANT
MUTANTES
MUTANTS
NUCLEOTIDE
NUCLEOTIDES
NUCLEOTIDOS
Plasmids
RNA
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Viability
Yeasts
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The structural gene for yeast DNA topoisomerase I (TOP1) has been cloned from two yeast genomic plasmid banks. Integration of a plasmid carrying the gene into the chromosome and subsequent genetic mapping shows that TOP1 is identical to the gene previously called MAK1. Seven top1 (mak1) mutants including gene disruptions are viable, demonstrating that DNA topoisomerase I is not essential for viability in yeast. A 3787-base-pair DNA fragment including the gene has been sequenced. The protein predicted from the DNA sequence has 769 amino acids and a molecular weight of 90,020.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.82.13.4374