Multistep Transformation by Defined Fragments of Herpes Simplex Virus Type 2 DNA: Oncogenic Region and Its Gene Product

Diploid Syrian hamster embryo cells transfected with Bgl II C fragment of herpes simplex virus type 2 DNA acquired a neoplastic phenotype. Cultures transfected with its left-hand 64% subclone EcoRI/HindIII fragment AE (0.419-0.525 map unit) grew into established but nontumorigenic lines. Transfectio...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1985-12, Vol.82 (24), p.8493-8497
Main Authors: Hayashi, Yoshinobu, Iwasaka, Tsuyoshi, Smith, Cynthia C., Aurelian, Laure, Lewis, George K., Paul O. P. Ts'o
Format: Article
Language:English
Subjects:
3T3 cells
Animals
Antigens, Viral, Tumor - genetics
Biological and medical sciences
Cell lines
Cell Survival
Cell Transformation, Viral
Cells
Cells, Cultured
Cellular immunity
Cricetinae
Diploidy
DNA
DNA, Viral - genetics
Embryonic cells
Fundamental and applied biological sciences. Psychology
Genetics
K562 cells
Messenger RNA
Microbiology
Molecular Weight
Oncogene Proteins, Viral - genetics
Oncogenes
Phenotype
Proteins
Ribonucleotide Reductases - genetics
Simplexvirus - genetics
Viral Proteins - genetics
Virology
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Summary:Diploid Syrian hamster embryo cells transfected with Bgl II C fragment of herpes simplex virus type 2 DNA acquired a neoplastic phenotype. Cultures transfected with its left-hand 64% subclone EcoRI/HindIII fragment AE (0.419-0.525 map unit) grew into established but nontumorigenic lines. Transfection of EcoRI/HindIII AE-immortalized cells with a 4.4-kilobase Sac I/BamHI subfragment within BamHI E (0.554-0.584 map unit; overlaps the right-hand 16% of BglII C) converted them to tumorigenicity. The 4.4-kilobase subfragment encodes a 144-kDa protein immunologically and structurally similar to an infected cell protein designated ICP 10. DNA extracted from cells transformed with the 4.4-kilobase subfragment exhibited discrete hybridizing bands homologous to BamHI E fragment. Monoclonal antibody to ICP 10 precipitated a 144-kDa protein from the transformed cells and stained them in immunofluorescence. A tumor derivative established with the transformed cells did not stain with this antibody, but ≈ 25% of the cells stained with a monoclonal antibody to c-myc protooncogene products.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.82.24.8493