Cholera Toxin Inhibits the T-Cell Antigen Receptor-Mediated Increases in Inositol Trisphosphate and Cytoplasmic Free Calcium

The addition of monoclonal antibodies to the antigen receptor complex on the malignant human T-cell line Jurkat generates increases in inositol trisphosphate and in the concentration of cytoplasmic free calcium. Exposure of Jurkat cells to cholera toxin for 3 hr inhibited these receptor-mediated eve...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1986-08, Vol.83 (15), p.5673-5677
Main Authors: Imboden, John B., Shoback, Dolores M., Pattison, Gregory, Stobo, John D.
Format: Article
Language:English
Subjects:
Adenosine Diphosphate Ribose - metabolism
Antibodies, Monoclonal
Antigen receptors
Antigens
Bacteriology
Biological and medical sciences
Calcium - physiology
Cell Line
Cholera
Cholera Toxin - pharmacology
Cyclic AMP - physiology
Cytoplasm - physiology
Fluorescence
Fundamental and applied biological sciences. Psychology
GTP-Binding Proteins - metabolism
Humans
Inositol Phosphates - physiology
Inositols
Kinetics
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Physiological regulation
Receptors
Receptors, Antigen, T-Cell - physiology
Sugar Phosphates - physiology
T lymphocytes
T-Lymphocytes - immunology
T-Lymphocytes - physiology
Toxins
Vibrio cholerae
Whooping cough
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The addition of monoclonal antibodies to the antigen receptor complex on the malignant human T-cell line Jurkat generates increases in inositol trisphosphate and in the concentration of cytoplasmic free calcium. Exposure of Jurkat cells to cholera toxin for 3 hr inhibited these receptor-mediated events and led to a selective, partial loss of the antigen receptor complex from the cellular surface. None of the effects of cholera toxin on the antigen receptor complex were mimicked by the B subunit of cholera toxin or by increasing intracellular cAMP levels with either forskolin or 8-bromo cAMP. These results suggest that a cholera toxin substrate can regulate signal transduction by the T-cell antigen receptor.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.83.15.5673