Loading…

Multiple Calcium Channels Mediate Neurotransmitter Release from Peripheral Neurons

We examined the effects of dihydropyridine drugs on evoked neurotransmitter release from cultured neonatal rat sensory and sympathetic neurons. Depolarization with K+-rich solutions increased the release of substance P from cultured sensory neurons. This release was enhanced by BAY K8644 and (+)-202...

Full description

Saved in:

Bibliographic Details
Published in:	Proceedings of the National Academy of Sciences - PNAS 1986-09, Vol.83 (17), p.6656-6659
Main Authors:	Perney, Teresa M., Hirning, Lane D., Leeman, Susan E., Miller, Richard J.
Format:	Article
Language:	English
Subjects:	Agonists Animals Biological and medical sciences Calcium - physiology Calcium Channel Blockers - pharmacology Calcium channels Cell physiology Culture Techniques Cultured cells Dihydropyridines Fundamental and applied biological sciences. Psychology Ganglia, Spinal - physiology Ganglia, Sympathetic - physiology Ion Channels - physiology Molecular and cellular biology Nerves Neurons Neuroscience Neurotransmission Neurotransmitter Agents - metabolism Neurotransmitters Norepinephrine - metabolism Peripheral Nerves - physiology Potassium - physiology Rats Sensory neurons Substance P - metabolism Transmitters
Citations:	Items that cite this one
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

cited_by	cdi_FETCH-LOGICAL-c556t-b17c1178770dc28d2c982353cd8cf76aacb089456cbad15a37218e21467d886e3
cites
container_end_page	6659
container_issue	17
container_start_page	6656
container_title	Proceedings of the National Academy of Sciences - PNAS
container_volume	83
creator	Perney, Teresa M. Hirning, Lane D. Leeman, Susan E. Miller, Richard J.
description	We examined the effects of dihydropyridine drugs on evoked neurotransmitter release from cultured neonatal rat sensory and sympathetic neurons. Depolarization with K+-rich solutions increased the release of substance P from cultured sensory neurons. This release was enhanced by BAY K8644 and (+)-202791 and was blocked by a variety of other dihydropyridines including (-)-202791, by Co2+, or in Ca2+-free solutions. K+-rich solutions also stimulated the release of [3H]norepinephrine from cultured sympathetic neurons. This release was also completely blocked by Co2+ or in Ca2+-free solution. In contrast to the situation in sensory neurons, however, the evoked release of [3H]norepinephrine was completely resistant to the blocking effects of dihydropyridine such as nimodipine. However, BAY K8644 was able to enhance the evoked release of [3H]norepinephrine, and this enhancement was blocked by nimodipine. These results are discussed in relation to the possible participation of multiple types of calcium channels in the release of neurotransmitters.
doi_str_mv	10.1073/pnas.83.17.6656
format	article
fullrecord	<record><control><sourceid>jstor_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1073_pnas_83_17_6656</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>28417</jstor_id><sourcerecordid>28417</sourcerecordid><originalsourceid>FETCH-LOGICAL-c556t-b17c1178770dc28d2c982353cd8cf76aacb089456cbad15a37218e21467d886e3</originalsourceid><addsrcrecordid>eNp9kc1v1DAQxS0EKkvhjIQEygHRU7b-SOzJgQNa8SW1BVVwtrzOhHXlfGA7CP77erVhoZee5vB-b-bpDSHPGV0zqsT5NJi4BrFmai1lLR-QFaMNK2XV0IdkRSlXJVS8ekyexHhDKW1qoCfkhFccqGhW5Ppy9slNHouN8dbNfbHZmWFAH4tLbJ1JWFzhHMYUzBB7lxKG4ho9mohFF8a--IrBTTsMxh_AIT4ljzrjIz5b5in5_uH9t82n8uLLx8-bdxelrWuZyi1TljEFStHWcmi5bYCLWtgWbKekMXZLoalqabemZbURijNAziqpWgCJ4pS8Peyd5m2PrcUhh_R6Cq434Y8ejdN3lcHt9I_xlxaQmxLZ_2bxh_HnjDHp3kWL3psBxzlqVoHksqkyeH4AbRhjDNgdbzCq91_Q-y9oEJopvf9Cdrz8P9qRX2rP-utFN9Ea3-VyrYtHLJcCIFjGXi3Yfv9f9c6ds3sB3c3eJ_ydMvniQN7ENIZ_gaBiStwCDKOy5g</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14862694</pqid></control><display><type>article</type><title>Multiple Calcium Channels Mediate Neurotransmitter Release from Peripheral Neurons</title><source>NCBI_PubMed Central（免费）</source><source>JSTOR</source><creator>Perney, Teresa M. ; Hirning, Lane D. ; Leeman, Susan E. ; Miller, Richard J.</creator><creatorcontrib>Perney, Teresa M. ; Hirning, Lane D. ; Leeman, Susan E. ; Miller, Richard J.</creatorcontrib><description>We examined the effects of dihydropyridine drugs on evoked neurotransmitter release from cultured neonatal rat sensory and sympathetic neurons. Depolarization with K+-rich solutions increased the release of substance P from cultured sensory neurons. This release was enhanced by BAY K8644 and (+)-202791 and was blocked by a variety of other dihydropyridines including (-)-202791, by Co2+, or in Ca2+-free solutions. K+-rich solutions also stimulated the release of [3H]norepinephrine from cultured sympathetic neurons. This release was also completely blocked by Co2+ or in Ca2+-free solution. In contrast to the situation in sensory neurons, however, the evoked release of [3H]norepinephrine was completely resistant to the blocking effects of dihydropyridine such as nimodipine. However, BAY K8644 was able to enhance the evoked release of [3H]norepinephrine, and this enhancement was blocked by nimodipine. These results are discussed in relation to the possible participation of multiple types of calcium channels in the release of neurotransmitters.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.83.17.6656</identifier><identifier>PMID: 2428039</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Agonists ; Animals ; Biological and medical sciences ; Calcium - physiology ; Calcium Channel Blockers - pharmacology ; Calcium channels ; Cell physiology ; Culture Techniques ; Cultured cells ; Dihydropyridines ; Fundamental and applied biological sciences. Psychology ; Ganglia, Spinal - physiology ; Ganglia, Sympathetic - physiology ; Ion Channels - physiology ; Molecular and cellular biology ; Nerves ; Neurons ; Neuroscience ; Neurotransmission ; Neurotransmitter Agents - metabolism ; Neurotransmitters ; Norepinephrine - metabolism ; Peripheral Nerves - physiology ; Potassium - physiology ; Rats ; Sensory neurons ; Substance P - metabolism ; Transmitters</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1986-09, Vol.83 (17), p.6656-6659</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c556t-b17c1178770dc28d2c982353cd8cf76aacb089456cbad15a37218e21467d886e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/83/17.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/28417$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/28417$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793,58238,58471</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7878831$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2428039$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Perney, Teresa M.</creatorcontrib><creatorcontrib>Hirning, Lane D.</creatorcontrib><creatorcontrib>Leeman, Susan E.</creatorcontrib><creatorcontrib>Miller, Richard J.</creatorcontrib><title>Multiple Calcium Channels Mediate Neurotransmitter Release from Peripheral Neurons</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We examined the effects of dihydropyridine drugs on evoked neurotransmitter release from cultured neonatal rat sensory and sympathetic neurons. Depolarization with K+-rich solutions increased the release of substance P from cultured sensory neurons. This release was enhanced by BAY K8644 and (+)-202791 and was blocked by a variety of other dihydropyridines including (-)-202791, by Co2+, or in Ca2+-free solutions. K+-rich solutions also stimulated the release of [3H]norepinephrine from cultured sympathetic neurons. This release was also completely blocked by Co2+ or in Ca2+-free solution. In contrast to the situation in sensory neurons, however, the evoked release of [3H]norepinephrine was completely resistant to the blocking effects of dihydropyridine such as nimodipine. However, BAY K8644 was able to enhance the evoked release of [3H]norepinephrine, and this enhancement was blocked by nimodipine. These results are discussed in relation to the possible participation of multiple types of calcium channels in the release of neurotransmitters.</description><subject>Agonists</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium - physiology</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Calcium channels</subject><subject>Cell physiology</subject><subject>Culture Techniques</subject><subject>Cultured cells</subject><subject>Dihydropyridines</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ganglia, Spinal - physiology</subject><subject>Ganglia, Sympathetic - physiology</subject><subject>Ion Channels - physiology</subject><subject>Molecular and cellular biology</subject><subject>Nerves</subject><subject>Neurons</subject><subject>Neuroscience</subject><subject>Neurotransmission</subject><subject>Neurotransmitter Agents - metabolism</subject><subject>Neurotransmitters</subject><subject>Norepinephrine - metabolism</subject><subject>Peripheral Nerves - physiology</subject><subject>Potassium - physiology</subject><subject>Rats</subject><subject>Sensory neurons</subject><subject>Substance P - metabolism</subject><subject>Transmitters</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNp9kc1v1DAQxS0EKkvhjIQEygHRU7b-SOzJgQNa8SW1BVVwtrzOhHXlfGA7CP77erVhoZee5vB-b-bpDSHPGV0zqsT5NJi4BrFmai1lLR-QFaMNK2XV0IdkRSlXJVS8ekyexHhDKW1qoCfkhFccqGhW5Ppy9slNHouN8dbNfbHZmWFAH4tLbJ1JWFzhHMYUzBB7lxKG4ho9mohFF8a--IrBTTsMxh_AIT4ljzrjIz5b5in5_uH9t82n8uLLx8-bdxelrWuZyi1TljEFStHWcmi5bYCLWtgWbKekMXZLoalqabemZbURijNAziqpWgCJ4pS8Peyd5m2PrcUhh_R6Cq434Y8ejdN3lcHt9I_xlxaQmxLZ_2bxh_HnjDHp3kWL3psBxzlqVoHksqkyeH4AbRhjDNgdbzCq91_Q-y9oEJopvf9Cdrz8P9qRX2rP-utFN9Ea3-VyrYtHLJcCIFjGXi3Yfv9f9c6ds3sB3c3eJ_ydMvniQN7ENIZ_gaBiStwCDKOy5g</recordid><startdate>19860901</startdate><enddate>19860901</enddate><creator>Perney, Teresa M.</creator><creator>Hirning, Lane D.</creator><creator>Leeman, Susan E.</creator><creator>Miller, Richard J.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>5PM</scope></search><sort><creationdate>19860901</creationdate><title>Multiple Calcium Channels Mediate Neurotransmitter Release from Peripheral Neurons</title><author>Perney, Teresa M. ; Hirning, Lane D. ; Leeman, Susan E. ; Miller, Richard J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c556t-b17c1178770dc28d2c982353cd8cf76aacb089456cbad15a37218e21467d886e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Agonists</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium - physiology</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Calcium channels</topic><topic>Cell physiology</topic><topic>Culture Techniques</topic><topic>Cultured cells</topic><topic>Dihydropyridines</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Ganglia, Spinal - physiology</topic><topic>Ganglia, Sympathetic - physiology</topic><topic>Ion Channels - physiology</topic><topic>Molecular and cellular biology</topic><topic>Nerves</topic><topic>Neurons</topic><topic>Neuroscience</topic><topic>Neurotransmission</topic><topic>Neurotransmitter Agents - metabolism</topic><topic>Neurotransmitters</topic><topic>Norepinephrine - metabolism</topic><topic>Peripheral Nerves - physiology</topic><topic>Potassium - physiology</topic><topic>Rats</topic><topic>Sensory neurons</topic><topic>Substance P - metabolism</topic><topic>Transmitters</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Perney, Teresa M.</creatorcontrib><creatorcontrib>Hirning, Lane D.</creatorcontrib><creatorcontrib>Leeman, Susan E.</creatorcontrib><creatorcontrib>Miller, Richard J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Perney, Teresa M.</au><au>Hirning, Lane D.</au><au>Leeman, Susan E.</au><au>Miller, Richard J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiple Calcium Channels Mediate Neurotransmitter Release from Peripheral Neurons</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1986-09-01</date><risdate>1986</risdate><volume>83</volume><issue>17</issue><spage>6656</spage><epage>6659</epage><pages>6656-6659</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>We examined the effects of dihydropyridine drugs on evoked neurotransmitter release from cultured neonatal rat sensory and sympathetic neurons. Depolarization with K+-rich solutions increased the release of substance P from cultured sensory neurons. This release was enhanced by BAY K8644 and (+)-202791 and was blocked by a variety of other dihydropyridines including (-)-202791, by Co2+, or in Ca2+-free solutions. K+-rich solutions also stimulated the release of [3H]norepinephrine from cultured sympathetic neurons. This release was also completely blocked by Co2+ or in Ca2+-free solution. In contrast to the situation in sensory neurons, however, the evoked release of [3H]norepinephrine was completely resistant to the blocking effects of dihydropyridine such as nimodipine. However, BAY K8644 was able to enhance the evoked release of [3H]norepinephrine, and this enhancement was blocked by nimodipine. These results are discussed in relation to the possible participation of multiple types of calcium channels in the release of neurotransmitters.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2428039</pmid><doi>10.1073/pnas.83.17.6656</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record>
fulltext	fulltext
identifier	ISSN: 0027-8424
ispartof	Proceedings of the National Academy of Sciences - PNAS, 1986-09, Vol.83 (17), p.6656-6659
issn	0027-8424 1091-6490
language	eng
recordid	cdi_crossref_primary_10_1073_pnas_83_17_6656
source	NCBI_PubMed Central（免费）; JSTOR
subjects	Agonists Animals Biological and medical sciences Calcium - physiology Calcium Channel Blockers - pharmacology Calcium channels Cell physiology Culture Techniques Cultured cells Dihydropyridines Fundamental and applied biological sciences. Psychology Ganglia, Spinal - physiology Ganglia, Sympathetic - physiology Ion Channels - physiology Molecular and cellular biology Nerves Neurons Neuroscience Neurotransmission Neurotransmitter Agents - metabolism Neurotransmitters Norepinephrine - metabolism Peripheral Nerves - physiology Potassium - physiology Rats Sensory neurons Substance P - metabolism Transmitters
title	Multiple Calcium Channels Mediate Neurotransmitter Release from Peripheral Neurons
url	http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T09%3A26%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Multiple%20Calcium%20Channels%20Mediate%20Neurotransmitter%20Release%20from%20Peripheral%20Neurons&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Perney,%20Teresa%20M.&rft.date=1986-09-01&rft.volume=83&rft.issue=17&rft.spage=6656&rft.epage=6659&rft.pages=6656-6659&rft.issn=0027-8424&rft.eissn=1091-6490&rft.coden=PNASA6&rft_id=info:doi/10.1073/pnas.83.17.6656&rft_dat=%3Cjstor_cross%3E28417%3C/jstor_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c556t-b17c1178770dc28d2c982353cd8cf76aacb089456cbad15a37218e21467d886e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=14862694&rft_id=info:pmid/2428039&rft_jstor_id=28417&rfr_iscdi=true