A Protein Kinase Antigenically Related to pp60v-src Possibly Involved in Yeast Cell Cycle Control: Positive in vivo Regulation by Sterol

The effects of ergosterol, yeast's natural sterol, on cell cycling and a protein kinase antigenically related to pp60v-src were examined in a sterol auxotroph of Saccharomyces cerevisiae. Sterol-depleted cells accumulate in an unbudded, G1 state. Cell budding and proliferation are reinitiated u...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-06, Vol.84 (12), p.4012-4016
Main Authors: Dahl, Charles, Biemann, Hans-Peter, Dahl, Jean
Format: Article
Language:English
Subjects:
3T3 cells
B lymphocytes
Biological and medical sciences
Budding
Cell Cycle
Cell growth
Cell Membrane - enzymology
Cholesterols
Cycloheximide - pharmacology
Epitopes - analysis
Ergosterol - pharmacology
Ergosterol - physiology
Fundamental and applied biological sciences. Psychology
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Interphase
Kinetics
Microbiology
Mycology
Oncogene Protein pp60(v-src)
Phosphatidylinositols
Protein Kinases - immunology
Protein Kinases - metabolism
Retroviridae Proteins - immunology
Retroviridae Proteins - metabolism
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - enzymology
Sterols
Yeasts
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Summary:The effects of ergosterol, yeast's natural sterol, on cell cycling and a protein kinase antigenically related to pp60v-src were examined in a sterol auxotroph of Saccharomyces cerevisiae. Sterol-depleted cells accumulate in an unbudded, G1 state. Cell budding and proliferation are reinitiated upon addition of nonlimiting ergosterol or cholesterol with trace ergosterol, whereas cholesterol or trace ergosterol alone is less effective. Stimulation of a protein kinase associated with immune complexes of yeast protein and anti-pp60v-src shows a positive correlation with exit from the G1 phase following ergosterol addition. Ergosterol-stimulated cells also demonstrate an increase in phosphatidylinositol kinase activity. The data suggest that hormonal levels of ergosterol (effective concentration, ≈ 1 nM) participate in a signaling process associated with a protein kinase possibly involved in yeast cell cycle control.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.12.4012